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Literature summary extracted from

  • Miyazaki, S.S.; Toki, S.; Izumi, Y.; Yamada, H.
    Purification and characterization of a serine hydroxymethyltransferase from an obligate methylotroph, Hyphomicrobium methylovorum GM2 (1987), Eur. J. Biochem., 162, 533-540.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 1,10-phenanthroline
-
 Hyphomicrobium methylovorum
2.1.2.1 2,2'-dipyridyl
-
 Hyphomicrobium methylovorum
2.1.2.1 5,5-dithiobis(2-nitrobenzoic acid)
-
 Hyphomicrobium methylovorum
2.1.2.1 Ag+ strong Hyphomicrobium methylovorum
2.1.2.1 Cu2+ strong Hyphomicrobium methylovorum
2.1.2.1 D-cycloserine
-
 Hyphomicrobium methylovorum
2.1.2.1 Hg2+ strong Hyphomicrobium methylovorum
2.1.2.1 KCl
-
 Hyphomicrobium methylovorum
2.1.2.1 KCN
-
 Hyphomicrobium methylovorum
2.1.2.1 LiCl
-
 Hyphomicrobium methylovorum
2.1.2.1 additional information not: EDTA Hyphomicrobium methylovorum
2.1.2.1 NaCl
-
 Hyphomicrobium methylovorum
2.1.2.1 NaF
-
 Hyphomicrobium methylovorum
2.1.2.1 NH2OH
-
 Hyphomicrobium methylovorum
2.1.2.1 Pb2+ strong Hyphomicrobium methylovorum
2.1.2.1 PCMB strong Hyphomicrobium methylovorum
2.1.2.1 Penicillamine
-
 Hyphomicrobium methylovorum
2.1.2.1 phenylhydrazine partially reversible by pyridoxal 5'-phosphate Hyphomicrobium methylovorum
2.1.2.1 Semicarbazide
-
 Hyphomicrobium methylovorum
2.1.2.1 Sodium borohydride
-
 Hyphomicrobium methylovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information
-
 Hyphomicrobium methylovorum
2.1.2.1 0.04 0.046 tetrahydrofolate glycine Hyphomicrobium methylovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 48000
-
 2 * 48000, SDS-PAGE Hyphomicrobium methylovorum
2.1.2.1 80000
-
 HPLC gel filtration Hyphomicrobium methylovorum
2.1.2.1 98000
-
 gel filtration Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Hyphomicrobium methylovorum key enzyme of serine pathway for assimilation of C1-compounds glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Hyphomicrobium methylovorum
-
 strain GM2, a glycine resistant mutant derived from strain KM146
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 7.9
-
 serine synthesis Hyphomicrobium methylovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Hyphomicrobium methylovorum glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r
2.1.2.1 L-serine + tetrahydrofolate key enzyme of serine pathway for assimilation of C1-compounds Hyphomicrobium methylovorum glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer 2 * 48000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 37
-
 serine synthesis Hyphomicrobium methylovorum
2.1.2.1 37 40 3-phenylserine degradation Hyphomicrobium methylovorum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.2.1 29 42 serine synthesis, about half-maximal activity at 28.5°C and 90% of maximal activity at 42°C Hyphomicrobium methylovorum
2.1.2.1 30 42 2-phenylserine degradation, about half-maximal activity at 29.5°C and 80% of maximal activity at 42°C Hyphomicrobium methylovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 40
-
 below, at least 10 min stable Hyphomicrobium methylovorum
2.1.2.1 45
-
 above, rapid loss of activity Hyphomicrobium methylovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 additional information
-
-
 Hyphomicrobium methylovorum
2.1.2.1 7.5 8 3-phenylserine degradation Hyphomicrobium methylovorum
2.1.2.1 8
-
 serine synthesis Hyphomicrobium methylovorum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.1 7 9 serine synthesis: about 80% of maximal activity at pH 7.0 and 9.0 Hyphomicrobium methylovorum
2.1.2.1 7.2 8.6 3-phenylserine degradation: about half-maximal activity at pH 7.2 and 8.6 Hyphomicrobium methylovorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.2.1 6 7.5 stable Hyphomicrobium methylovorum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate 2 mol per mol enzyme Hyphomicrobium methylovorum
2.1.2.1 tetrahydrofolate requirement with L-serine or L-2-methylserine as substrate Hyphomicrobium methylovorum