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Literature summary extracted from

  • Jagath-Reddy, J.; Ganesan, K.; Savithri, H.S.; Datta, A.; Rao, N.A.
    cDNA cloning, overexpression in Escherichia coli, purification and characterization of sheep liver cytosolic serine hydroxymethyltransferase (1995), Eur. J. Biochem., 230, 533-537.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expression in Escherichia coli Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 D-alanine
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.82
-
 tetrahydrofolate recombinant enzyme Ovis aries
2.1.2.1 1
-
 L-serine recombinant enzyme Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytoplasm
-
 Ovis aries 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 53000
-
 4 * 53000, SDS-PAGE Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Ovis aries enzyme is a component of thymidylate cycle glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 recombinant enzyme Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 additional information
-
-
 Ovis aries
2.1.2.1 0.02
-
 crude extract Ovis aries
2.1.2.1 0.6
-
 recombinant enzyme, crude extract Ovis aries
2.1.2.1 3.2
-
 purified recombinant enzyme Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Ovis aries glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r
2.1.2.1 L-serine + tetrahydrofolate enzyme is a component of thymidylate cycle Ovis aries glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homotetramer 4 * 53000, SDS-PAGE Ovis aries