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Literature summary extracted from

  • Axelrod, J.; Weissbach, H.
    Purification and properties of hydroxyindole-O-methyl transferase (1961), J. Biol. Chem., 236, 211-213.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.4 p-chloromercuribenzoate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.4 0.046
-
 S-adenosyl-L-methionine
-
 Bos taurus
2.1.1.4 0.054
-
 N-acetylserotonin
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.4 N-acetylserotonin + S-adenosyl-L-methionine Bos taurus formation of the hormone melatonin S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 N-acetylserotonin + S-adenosyl-L-methionine Felis catus formation of the hormone melatonin S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 N-acetylserotonin + S-adenosyl-L-methionine Macaca mulatta formation of the hormone melatonin S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.4 Bos taurus
-
-
-
2.1.1.4 Felis catus
-
-
-
2.1.1.4 Macaca mulatta
-
 monkey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.4
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.4 brain not Bos taurus
-
2.1.1.4 brain not Felis catus
-
2.1.1.4 brain not Macaca mulatta
-
2.1.1.4 pineal gland
-
 Bos taurus
-
2.1.1.4 pineal gland
-
 Felis catus
-
2.1.1.4 pineal gland
-
 Macaca mulatta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.4 additional information
-
 enzyme assay Bos taurus
2.1.1.4 additional information
-
 enzyme assay Felis catus
2.1.1.4 additional information
-
 enzyme assay Macaca mulatta

Storage Stability

EC Number Storage Stability Organism
2.1.1.4 frozen, stable for several weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.4 4-hydroxy-N-acetyltryptamine + S-adenosyl-L-methionine 8% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + N-acetyl-4-methoxytryptamine
-
 ?
2.1.1.4 5-hydroxyindoleacetic acid + S-adenosyl-L-methionine 12% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + 5-methoxyindoleacetic acid
-
 ?
2.1.1.4 bufotenine + S-adenosyl-L-methionine 14% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + N,N-dimethyl-5-hydroxytryptamine
-
 ?
2.1.1.4 additional information absolute requirement for S-adenosylmethionine Bos taurus ?
-
 ?
2.1.1.4 additional information absolute requirement for S-adenosylmethionine Felis catus ?
-
 ?
2.1.1.4 additional information absolute requirement for S-adenosylmethionine Macaca mulatta ?
-
 ?
2.1.1.4 N-acetylserotonin + S-adenosyl-L-methionine formation of the hormone melatonin Bos taurus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 N-acetylserotonin + S-adenosyl-L-methionine formation of the hormone melatonin Felis catus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 N-acetylserotonin + S-adenosyl-L-methionine formation of the hormone melatonin Macaca mulatta S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 N-methylserotonin + S-adenosyl-L-methionine 9% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + N-methyl-5-methoxytryptamine
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Bos taurus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Felis catus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Macaca mulatta S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
2.1.1.4 serotonin + S-adenosyl-L-methionine 7% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.4 7.5 8.3
-
 Bos taurus