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Literature summary extracted from

  • Rajagopalan, R.; Altekar, W.
    Characterisation and purification of ribulose-bisphosphate carboxylase from heterotrophically grown halophilic archaebacterium Haloferax mediterranei (1994), Eur. J. Biochem., 221, 863-869.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.39 (NH4)2SO4
-
 Haloferax mediterranei
4.1.1.39 GSH
-
 Haloferax mediterranei
4.1.1.39 iodoacetamide
-
 Haloferax mediterranei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.39 Cs+ 0.8 M KCl optimal, can be replaced by NaCl, CsCl, RbCl, NH4Cl, LiCl Haloferax mediterranei
4.1.1.39 K+ 0.8 M KCl optimal, can be replaced by NaCl, CsCl, RbCl, NH4Cl, LiCl Haloferax mediterranei
4.1.1.39 Li+ 0.8 M KCl optimal, can be replaced by NaCl, CsCl, RbCl, NH4Cl, LiCl Haloferax mediterranei
4.1.1.39 Na+ 0.8 M KCl optimal, can be replaced by NaCl, CsCl, RbCl, NH4Cl, LiCl Haloferax mediterranei
4.1.1.39 NH4+ 0.8 M KCl optimal, can be replaced by NaCl, CsCl, RbCl, NH4Cl, LiCl Haloferax mediterranei
4.1.1.39 Rb+ 0.8 M KCl optimal, can be replaced by NaCl, CsCl, RbCl, NH4Cl, LiCl Haloferax mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.39 14000
-
 L8S8, 8 * 56000 (L) + 8 * 14000 (S), SDS-PAGE Haloferax mediterranei
4.1.1.39 56000
-
 L8S8, 8 * 56000 (L) + 8 * 14000 (S), SDS-PAGE Haloferax mediterranei
4.1.1.39 500000
-
 sucrose density gradient centrifugation Haloferax mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2 Haloferax mediterranei
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Haloferax mediterranei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39
-
 Haloferax mediterranei

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.39 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction Haloferax mediterranei
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.39 0.052
-
 carboxylase Haloferax mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Haloferax mediterranei D-3-Phosphoglycerate
-
 ?
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Haloferax mediterranei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.39 hexadecamer L8S8, 8 * 56000 (L) + 8 * 14000 (S), SDS-PAGE Haloferax mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.39 30
-
-
 Haloferax mediterranei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.39 45
-
 diminished activity above Haloferax mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.39 7.8
-
-
 Haloferax mediterranei