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Literature summary extracted from

  • Stehlin, C.; Dahm, A.; Kirschner, K.
    Deletion mutagenesis as a test of evolutionary relatedness of indoleglycerol phosphate synthase with other TIM barrel enzymes (1997), FEBS Lett., 403, 268-272.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.48 L5V Leu5Val without effect on turnover-number and Km Escherichia coli
4.1.1.48 additional information
-
 Escherichia coli
4.1.1.48 additional information mutant enzyme with deletion of 8 terminal residues is soluble and has the same turnover number as the wild-type protein but a 220-fold greater Km-value Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.48 0.000045
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type enzyme Saccharolobus solfataricus
4.1.1.48 0.00021
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate mutant Leu5Val Escherichia coli
4.1.1.48 0.00034
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type enzyme Escherichia coli
4.1.1.48 0.0099
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate truncated mutant Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Escherichia coli
-
-
-
4.1.1.48 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Escherichia coli 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Saccharolobus solfataricus 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.48 0.033
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type enzyme Saccharolobus solfataricus
4.1.1.48 0.041
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate truncated mutant Saccharolobus solfataricus
4.1.1.48 2
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate mutant Leu5Val Escherichia coli
4.1.1.48 2.2
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type enzyme Escherichia coli