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Literature summary extracted from

  • Salvarrey, M.S.; Cannata, J.J.B.; Cazzulo, J.J.
    Phosphoenolpyruvate carboxykinase from the moderate halophile Vibrio costicola (1989), Biochem. J., 260, 221-230.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.49 dithiothreitol activates Salinivibrio costicola

General Stability

EC Number General Stability Organism
4.1.1.49 glycerol, 20% v/v, stabilizes against thermal inactivation at 30°C Salinivibrio costicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.49 Fluorescein mercuric acetate 0.0008 mM, 50% inhibition Salinivibrio costicola
4.1.1.49 iodoacetamide less than 10% inhibition Salinivibrio costicola
4.1.1.49 NEM less than 10% inhibition Salinivibrio costicola
4.1.1.49 o-Iodobenzoate 0.2 mM , 50% inhibition Salinivibrio costicola
4.1.1.49 p-chloromercuribenzenesulfonate 0.0025 mM, 50% inhibition Salinivibrio costicola
4.1.1.49 p-chloromercuribenzoate 0.007 mM, 50% inhibition Salinivibrio costicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.49 17
-
 CO2 CO2 in form of HCO3- Salinivibrio costicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.49 57000
-
 gel filtration Salinivibrio costicola
4.1.1.49 60000
-
 1 * 60000, SDS-PAGE Salinivibrio costicola

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.49 Salinivibrio costicola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.49
-
 Salinivibrio costicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.49 additional information
-
-
 Salinivibrio costicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Salinivibrio costicola ATP + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.49 monomer 1 * 60000, SDS-PAGE Salinivibrio costicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.49 30
-
 remaining activity after 214 h: 57% in presence of 20% v/v glycerol, 37% in presence of 1 mM NaCl, 31% in presence of 1 mM KCl, 28% in absence of salts Salinivibrio costicola

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.49 0.0008
-
 Fluorescein mercuric acetate
-
 Salinivibrio costicola
4.1.1.49 0.0025
-
 p-chloromercuribenzenesulfonate
-
 Salinivibrio costicola
4.1.1.49 0.007
-
 p-chloromercuribenzoate
-
 Salinivibrio costicola
4.1.1.49 0.2
-
 o-Iodobenzoate
-
 Salinivibrio costicola