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Literature summary extracted from

  • Rasmussen, A.M.; Gibson, R.M.M.; Godtfredsen, S.E.; Ottesen, M.
    Purification of alpha-acetolactate decarboxylase from Lactobacillus casei DSM 2547 (1985), Carlsberg Res. Commun., 50, 73-82.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.5 1,10-phenanthroline partial reactivation by Zn2+ Lacticaseibacillus casei
4.1.1.5 Cu2+
-
 Lacticaseibacillus casei
4.1.1.5 p-hydroxymercuribenzoate
-
 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.5 4.8
-
 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.5 27500
-
 2 * 27500, SDS-PAGE Lacticaseibacillus casei
4.1.1.5 48000
-
 gel filtration Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.5 Lacticaseibacillus casei
-
 strain DSM 2547
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.5
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lacticaseibacillus casei (R)-3-Hydroxybutan-2-one + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.5 dimer 2 * 27500, SDS-PAGE Lacticaseibacillus casei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.5 40
-
-
 Lacticaseibacillus casei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.5 37
-
 pH 5, half-life: 15 min. Bovine serum albumin, 1 mg/ml, increases half-life to 4.5 h Lacticaseibacillus casei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.5 5.57
-
 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.5 4.5 5
-
 Lacticaseibacillus casei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.5 4 7 pH 4.0: about 70% of maximal activity, pH 7.0: about 15% of maximal activity Lacticaseibacillus casei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.5 4 9 unstable below pH 4 and above pH 9 Lacticaseibacillus casei