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Literature summary extracted from

  • Hugo, E.R.; Byers, T.J.
    S-Adenosyl-L-methionine decarboxylase of Acanthamoeba castellanii (Neff): purification and properties (1993), Biochem. J., 295, 203-209.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.50 putrescine stimulation Acanthamoeba castellanii
4.1.1.50 pyruvate alpha-subunit contains an N-terminal pyruvoyl group Acanthamoeba castellanii
4.1.1.50 spermidine no effect Acanthamoeba castellanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.50 Berenil
-
 Acanthamoeba castellanii
4.1.1.50 Ethidium bromide
-
 Acanthamoeba castellanii
4.1.1.50 Hydroxystilbamidine
-
 Acanthamoeba castellanii
4.1.1.50 methylglyoxal bis(guanylhydrazone)
-
 Acanthamoeba castellanii
4.1.1.50 Pentamidine
-
 Acanthamoeba castellanii
4.1.1.50 Propamidine
-
 Acanthamoeba castellanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.50 0.03
-
 S-adenosyl-L-methionine in presence of 2 mM putrescine Acanthamoeba castellanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.50 14600
-
 2 * 32500, alpha, + 2 * 14600, beta, SDS-PAGE Acanthamoeba castellanii
4.1.1.50 32500
-
 2 * 32500, alpha, + 2 * 14600, beta, SDS-PAGE Acanthamoeba castellanii
4.1.1.50 88000
-
 gel filtration Acanthamoeba castellanii

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.50 Acanthamoeba castellanii
-
 Neff strain
-
4.1.1.50 Acanthamoeba castellanii NEFF
-
 Neff strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.50
-
 Acanthamoeba castellanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.50 0.158
-
-
 Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine
-
 Acanthamoeba castellanii (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
-
 ?
4.1.1.50 S-adenosyl-L-methionine
-
 Acanthamoeba castellanii NEFF (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.50 tetramer 2 * 32500, alpha, + 2 * 14600, beta, SDS-PAGE Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.50 7.2 7.8
-
 Acanthamoeba castellanii