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Literature summary extracted from

  • Nakazawa, T.; Hayashi, E.
    Phthalate and 4-hydroxyphthalate metabolism in Pseudomonas testosteroni: purification and properties of 4,5-dihydroxyphthalate decarboxylase (1978), Appl. Environ. Microbiol., 36, 264-269.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.1.1.55 no inactivation by dialysis against 0.2 mM of either EDTA or o-phenanthroline in 0.05 M Tris-acetate, pH 7.5, containing 0.2 mM dithiothreitol, at 4°C, overnight Comamonas testosteroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.55 2,4,6-Trinitrobenzene sulfonate inhibition is partially prevented by 4,5-dihydroxyphthalate or 4-hydroxyphthalate, 1 mM Comamonas testosteroni
4.1.1.55 3-hydroxybenzoate
-
 Comamonas testosteroni
4.1.1.55 3-methylcatechol weak Comamonas testosteroni
4.1.1.55 4-hydroxybenzoate
-
 Comamonas testosteroni
4.1.1.55 AgCl
-
 Comamonas testosteroni
4.1.1.55 anthranilate competitive Comamonas testosteroni
4.1.1.55 benzoate
-
 Comamonas testosteroni
4.1.1.55 gentisate weak Comamonas testosteroni
4.1.1.55 HgCl2
-
 Comamonas testosteroni
4.1.1.55 PCMB
-
 Comamonas testosteroni
4.1.1.55 Phthalate
-
 Comamonas testosteroni
4.1.1.55 protocatechuate
-
 Comamonas testosteroni
4.1.1.55 salicylate
-
 Comamonas testosteroni
4.1.1.55 terephthalate competitive Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.55 0.0105
-
 4,5-Dihydroxyphthalate
-
 Comamonas testosteroni
4.1.1.55 1.25
-
 4-Hydroxyphthalate
-
 Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.55 38000
-
 4 * 38000, SDS-PAGE Comamonas testosteroni
4.1.1.55 150000
-
 gel filtration Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.55 Comamonas testosteroni
-
-
-
4.1.1.55 Comamonas testosteroni NH1000
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.55
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.55 58.9
-
-
 Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
4.1.1.55 4°C, 0.05 M Tris-acetate, pH 7.5, 0.2 mM dithiothreitol, 50% loss of activity after 4 days Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.55 4,5-Dihydroxyphthalate
-
 Comamonas testosteroni 3,4-Dihydroxybenzoate + CO2
-
 ?
4.1.1.55 4,5-Dihydroxyphthalate
-
 Comamonas testosteroni NH1000 3,4-Dihydroxybenzoate + CO2
-
 ?
4.1.1.55 4-Hydroxyphthalate
-
 Comamonas testosteroni 3-Hydroxybenzoate
-
 ?
4.1.1.55 4-Hydroxyphthalate
-
 Comamonas testosteroni NH1000 3-Hydroxybenzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.55 tetramer 4 * 38000, SDS-PAGE Comamonas testosteroni

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.55 27
-
 pH 7.5, 3.5 h, about 20% loss of activity Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.55 7.5
-
-
 Comamonas testosteroni

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.55 7.5
-
 27°C, 3.5 h, about 20% loss of activity Comamonas testosteroni