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Literature summary extracted from

  • Zeida, M.; Wieser, M.; Yoshida, T.; Sugio, T.; Nagasawa, T.
    Purification and characterization of gallic acid decarboxylase from Pantoea agglomerans T71 (1998), Appl. Environ. Microbiol., 64, 4743-4747.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.1.59 synthesis synthesis of pyrogallol from readily available tannic acid by using the combined action of gallate decarboxylase and tannase in a two-enzyme resting cell bioconversion Pantoea agglomerans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.59 (NH4)2S2O8
-
 Pantoea agglomerans
4.1.1.59 1,10-phenanthroline
-
 Pantoea agglomerans
4.1.1.59 2,2'-bipyridyl
-
 Pantoea agglomerans
4.1.1.59 Ag2SO4
-
 Pantoea agglomerans
4.1.1.59 CuCl2
-
 Pantoea agglomerans
4.1.1.59 Cuprizone
-
 Pantoea agglomerans
4.1.1.59 EDTA
-
 Pantoea agglomerans
4.1.1.59 FeCl2 slight Pantoea agglomerans
4.1.1.59 FeCl3 slight Pantoea agglomerans
4.1.1.59 H2O2
-
 Pantoea agglomerans
4.1.1.59 HgCl2
-
 Pantoea agglomerans
4.1.1.59 K2CrO4
-
 Pantoea agglomerans
4.1.1.59 KCN
-
 Pantoea agglomerans
4.1.1.59 NaNO3
-
 Pantoea agglomerans
4.1.1.59 p-chloromercuribenzoate
-
 Pantoea agglomerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.59 0.96
-
 3,4,5-Trihydroxybenzoate
-
 Pantoea agglomerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.59 Iron required as cofactor, contains 0.8 mol of iron per mol of enzyme subunit Pantoea agglomerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.59 57000
-
 6 * 57000, SDS-PAGE Pantoea agglomerans
4.1.1.59 320000
-
 gel filtration Pantoea agglomerans

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.59 Pantoea agglomerans
-
 i.e. Enterobacter agglomerans, strain T71
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.1.59 oxygen-sensitive enzyme Pantoea agglomerans

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.59
-
 Pantoea agglomerans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.59 150
-
-
 Pantoea agglomerans

Storage Stability

EC Number Storage Stability Organism
4.1.1.59 -20°C, 50 mM potassium phosphate buffer, pH 6.0, containing glycerol and reducing agents, stable for 3 weeks Pantoea agglomerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.59 3,4,5-Trihydroxybenzoate reverse reaction not catalyzed Pantoea agglomerans Pyrogallol + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.59 hexamer 6 * 57000, SDS-PAGE Pantoea agglomerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.59 50
-
-
 Pantoea agglomerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.59 50
-
 stable below Pantoea agglomerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.59 6
-
-
 Pantoea agglomerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.59 6 10 stable in the range Pantoea agglomerans