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Literature summary extracted from

  • Kurosaki, F.
    Partial purification and characterization of 6-hydroxymellein-O-methyltransferase from elicitor-treated carrot cells (1996), Phytochemistry, 41, 1023-1027.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.108 6-methoxymellein
-
Daucus carota
2.1.1.108 Co2+
-
Daucus carota
2.1.1.108 Cu2+
-
Daucus carota
2.1.1.108 Fe2+
-
Daucus carota
2.1.1.108 Hg2+
-
Daucus carota
2.1.1.108 iodoacetoamide
-
Daucus carota
2.1.1.108 Mn2+
-
Daucus carota
2.1.1.108 p-chloromercuribenzoate
-
Daucus carota
2.1.1.108 S-adenosyl-L-homocysteine
-
Daucus carota
2.1.1.108 sulfhydryl reagents
-
Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.108 76000
-
gel filtration Daucus carota

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.108 S-adenosyl-L-methionine + 6-hydroxymellein Daucus carota enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue S-adenosyl-L-homocysteine + 6-methoxymellein
-
?
2.1.1.108 S-adenosyl-L-methionine + 6-hydroxymellein Daucus carota biosynthetic pathway S-adenosyl-L-homocysteine + 6-methoxymellein
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.108 Daucus carota
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.108 partial Daucus carota

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.108 S-adenosyl-L-methionine + 6-hydroxymellein = S-adenosyl-L-homocysteine + 6-methoxymellein mechanism Daucus carota

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.108 additional information
-
-
Daucus carota

Storage Stability

EC Number Storage Stability Organism
2.1.1.108 4°C, 7 d, complete loss of activity Daucus carota
2.1.1.108 frozen overnight, complete loss of activity Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.108 S-adenosyl-L-methionine + 6-hydroxymellein
-
Daucus carota S-adenosyl-L-homocysteine + 6-methoxymellein
-
?
2.1.1.108 S-adenosyl-L-methionine + 6-hydroxymellein enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue Daucus carota S-adenosyl-L-homocysteine + 6-methoxymellein
-
?
2.1.1.108 S-adenosyl-L-methionine + 6-hydroxymellein biosynthetic pathway Daucus carota S-adenosyl-L-homocysteine + 6-methoxymellein
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.108 7.5 8 pI: 5.7 Daucus carota

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.108 0.026
-
S-adenosyl-L-homocysteine
-
Daucus carota
2.1.1.108 0.047
-
6-methoxymellein
-
Daucus carota