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Literature summary extracted from

  • Keller, N.P.; Dischinger, H.C.; Bhatnagar, D.; Cleveland, T.E.; Ullah, A.H.J.
    Purification of a 40-kilodalton methyltransferase active in the aflatoxin biosynthetic pathway (1993), Appl. Environ. Microbiol., 59, 479-484.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.110 0.002
-
 sterigmatocystin
-
 Aspergillus parasiticus
2.1.1.110 0.0096
-
 S-adenosyl-L-methionine
-
 Aspergillus parasiticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.110 soluble
-
 Aspergillus parasiticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.110 40000
-
 x * 40000, SRRC 163, SDS-PAGE, the 40000 Da protein is distinct from the 168000 Da methyl transferase purified from the same fungal strain Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin Aspergillus flavus penultimate step in aflatoxin biosynthetic pathway S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin Aspergillus parasiticus penultimate step in aflatoxin biosynthetic pathway S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin Aspergillus parasiticus SRCC 163 penultimate step in aflatoxin biosynthetic pathway S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.110 Aspergillus flavus
-
-
-
2.1.1.110 Aspergillus parasiticus
-
 SRCC 163
-
2.1.1.110 Aspergillus parasiticus SRCC 163
-
 SRCC 163
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.110 strain SRRC 163, anion exchange, hydroxylapatite, DEAE-Sperodex, octyl avidgel, anion-exchange Aspergillus parasiticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.110 mycelium
-
 Aspergillus flavus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.110 0.021
-
-
 Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin
-
 Aspergillus flavus S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin
-
 Aspergillus parasiticus S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin penultimate step in aflatoxin biosynthetic pathway Aspergillus flavus S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin penultimate step in aflatoxin biosynthetic pathway Aspergillus parasiticus S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin
-
 Aspergillus parasiticus SRCC 163 S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin penultimate step in aflatoxin biosynthetic pathway Aspergillus parasiticus SRCC 163 S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.110 ? x * 40000, SRRC 163, SDS-PAGE, the 40000 Da protein is distinct from the 168000 Da methyl transferase purified from the same fungal strain Aspergillus parasiticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.110 40 45
-
 Aspergillus parasiticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.110 25 60 approx. 35% of maximal activity at 25°C, approx. 30% of maximal activity at 60°C Aspergillus parasiticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.110 1.4
-
 sterigmatocystin
-
 Aspergillus parasiticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.110 7 9.4
-
 Aspergillus parasiticus