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Literature summary extracted from

  • Takata, Y.; Konishi, K.; Gomi, T.; Fujioka, M.
    Rat guanidinoacetate methyltransferase. Effect of site-directed alteration of an aspartic acid residue that is conserved across most mammalian S-adenosylmethionine-dependent methyltransferases (1994), J. Biol. Chem., 269, 5537-5542.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.2 D129A active enzyme Rattus norvegicus
2.1.1.2 D129N active enzyme Rattus norvegicus
2.1.1.2 D134A inactive Rattus norvegicus
2.1.1.2 D134E lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 D134N much lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 T135A lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 Y133F lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 Y136F higher turnover than wild type enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.2 additional information
-
 additional information all mutant enzymes listed with Km Rattus norvegicus
2.1.1.2 0.007
-
 S-adenosyl-L-methionine wild type enzyme Rattus norvegicus
2.1.1.2 0.011
-
 Guanidinoacetate wild type enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate Rattus norvegicus
-
 S-adenosyl-L-homocysteine + creatine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.2 Rattus norvegicus P10868
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.2
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate
-
 Rattus norvegicus S-adenosyl-L-homocysteine + creatine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.2 additional information
-
 additional information all mutant enzymes listed with turnover Rattus norvegicus
2.1.1.2 0.081
-
 Guanidinoacetate wild type enzyme Rattus norvegicus