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Literature summary extracted from

  • Roy, P.H.; Weissbach, A.
    DNA methylase from HeLa cell nuclei (1975), Nucleic Acids Res., 2, 1669-1684.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.1.1.37 glycerol stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.37 iodoacetamide
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.37 0.00325
-
 S-adenosyl-L-methionine
-
 Homo sapiens
2.1.1.37 0.086
-
 Micrococcus luteus DNA containing cytosine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.37 nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.37 120000
-
 glycerol density gradient sedimentation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.37
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.37 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.37 0.000013
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.1.1.37 -70°C, stable for at least several weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + DNA
-
 Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA the enzyme is able to methylate homologues HeLa DNA, altough to a lesser extent than heterologous DNA. The enzyme can methylate single-stranded DNA three times higher than that of the corresponding double-stranded DNA, the 5' neighbor can be either G or C while the 3' neighbor is always G, enzyme can methylate poly(dG,dC) Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + Micrococcus luteus DNA containing cytosine
-
 Homo sapiens S-adenosyl-L-homocysteine + Micrococcus luteus DNA containing 5-methylcytosine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.37 6.5
-
-
 Homo sapiens