BRENDA - Enzyme Database

Biosynthesis of cyclitols. 23. A soluble enzyme from pea seedlings methylating myo-inositol to D-bornesitol

Wagner, I.; Hofmann, H.; Hoffmann-Ostenhof, O.; Hoppe-Seyler's Z. Physiol. Chem. 350, 1460-1464 (1969)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.1.1.40
glutathione stabilizes slightly
Pisum sativum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.40
iodoacetamide
-
Pisum sativum
2.1.1.40
N-ethylmaleimide
-
Pisum sativum
2.1.1.40
p-chloromercuribenzoate
-
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.40
2.3
-
myo-inositol
-
Pisum sativum
2.1.1.40
3.2
-
S-adenosyl-L-methionine
-
Pisum sativum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.40
30000
-
gel filtration
Pisum sativum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.40
S-adenosyl-L-methionine + myo-inositol
Pisum sativum
biosynthesis of cyclitols
S-adenosyl-L-homocysteine + D-bornesitol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.40
Pisum sativum
-
pea
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.40
partial
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.40
seedling
-
Pisum sativum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.40
D-bornesitol + S-adenosyl-L-methionine
low reaction rate
485318
Pisum sativum
1,3-di-O-methyl-myo-inositol + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.40
D-chiro-inositol + S-adenosyl-L-methionine
-
485318
Pisum sativum
quebrachitol + S-adenosyl-L-homocysteine
-
485318
Pisum sativum
?
2.1.1.40
L-bornesitol + S-adenosyl-L-methionine
low reaction rate
485318
Pisum sativum
1,3-di-O-methyl-myo-inositol + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.40
L-chiro-inositol + S-adenosyl-L-methionine
-
485318
Pisum sativum
quebrachitol + pinitol + S-adenosyl-L-homocysteine
-
485318
Pisum sativum
?
2.1.1.40
S-adenosyl-L-methionine + myo-inositol
-
485318
Pisum sativum
S-adenosyl-L-homocysteine + D-bornesitol
-
485318
Pisum sativum
?
2.1.1.40
S-adenosyl-L-methionine + myo-inositol
biosynthesis of cyclitols
485318
Pisum sativum
S-adenosyl-L-homocysteine + D-bornesitol
-
-
-
?
2.1.1.40
scyllo-inositol + S-adenosyl-L-methionine
low reaction rate
485318
Pisum sativum
?
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.1.1.40
3
-
24 h, 40% loss of activity
Pisum sativum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.40
7
-
-
Pisum sativum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.40
6.7
7.6
about 50% of activity maximum at pH 6.7 and 7.6
Pisum sativum
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.1.40
glutathione stabilizes slightly
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.40
iodoacetamide
-
Pisum sativum
2.1.1.40
N-ethylmaleimide
-
Pisum sativum
2.1.1.40
p-chloromercuribenzoate
-
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.40
2.3
-
myo-inositol
-
Pisum sativum
2.1.1.40
3.2
-
S-adenosyl-L-methionine
-
Pisum sativum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.40
30000
-
gel filtration
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.40
S-adenosyl-L-methionine + myo-inositol
Pisum sativum
biosynthesis of cyclitols
S-adenosyl-L-homocysteine + D-bornesitol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.40
partial
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.40
seedling
-
Pisum sativum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.40
D-bornesitol + S-adenosyl-L-methionine
low reaction rate
485318
Pisum sativum
1,3-di-O-methyl-myo-inositol + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.40
D-chiro-inositol + S-adenosyl-L-methionine
-
485318
Pisum sativum
quebrachitol + S-adenosyl-L-homocysteine
-
485318
Pisum sativum
?
2.1.1.40
L-bornesitol + S-adenosyl-L-methionine
low reaction rate
485318
Pisum sativum
1,3-di-O-methyl-myo-inositol + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.40
L-chiro-inositol + S-adenosyl-L-methionine
-
485318
Pisum sativum
quebrachitol + pinitol + S-adenosyl-L-homocysteine
-
485318
Pisum sativum
?
2.1.1.40
S-adenosyl-L-methionine + myo-inositol
-
485318
Pisum sativum
S-adenosyl-L-homocysteine + D-bornesitol
-
485318
Pisum sativum
?
2.1.1.40
S-adenosyl-L-methionine + myo-inositol
biosynthesis of cyclitols
485318
Pisum sativum
S-adenosyl-L-homocysteine + D-bornesitol
-
-
-
?
2.1.1.40
scyllo-inositol + S-adenosyl-L-methionine
low reaction rate
485318
Pisum sativum
?
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.1.1.40
3
-
24 h, 40% loss of activity
Pisum sativum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.40
7
-
-
Pisum sativum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.40
6.7
7.6
about 50% of activity maximum at pH 6.7 and 7.6
Pisum sativum