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Literature summary extracted from

  • Fang, J.; Feng, Q.; Ketel, C.S.; Wang, H.; Cao, R.; Xia, L.; Erdjument-Bromage, H.; Tempst, P.; Simon, J.A.; Zhang, Y.
    Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase (2002), Curr. Biol., 12, 1086-1099.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.362 glutathione-S-transferase-fusion protein, SET gene Homo sapiens
2.1.1.362 glutathione-S-transferase-fusion protein, SET gene Drosophila elegans

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.362 H299A overlap PCR, destroyed SET gene, no activity remaining Drosophila elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.362 nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.362 45000
-
 SDS-PAGE Homo sapiens
2.1.1.362 45000
-
 SDS-PAGE Drosophila elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.362 additional information Drosophila melanogaster
-
 ?
-
 ?
2.1.1.362 additional information Homo sapiens histone methylation has significant effects on heterochromatin formation and transcriptional regulation ?
-
 ?
2.1.1.362 additional information Drosophila elegans histone methylation has significant effects on heterochromatin formation and transcriptional regulation ?
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine Drosophila melanogaster
-
 S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine Homo sapiens Lys20 of histone 4, SET8 S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine Drosophila elegans Lys20 of histone 4, SET8 S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.362 Drosophila elegans
-
-
-
2.1.1.362 Drosophila melanogaster
-
 SET8 sequence
-
2.1.1.362 Homo sapiens Q9NQR1 HeLa cells
-
2.1.1.362 Homo sapiens Q9NQR1 SET8 sequence
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.362 partial Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine amino acid sequence comparison Homo sapiens
a
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine amino acid sequence comparison Drosophila elegans
a
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence Drosophila melanogaster
a
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence Homo sapiens
a
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence Drosophila elegans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.362 additional information
-
 Drosophila melanogaster ?
-
 ?
2.1.1.362 additional information histone methylation has significant effects on heterochromatin formation and transcriptional regulation Homo sapiens ?
-
 ?
2.1.1.362 additional information histone methylation has significant effects on heterochromatin formation and transcriptional regulation Drosophila elegans ?
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine
-
 Drosophila elegans S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine high substrate and methylation site specificity Drosophila melanogaster S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine high substrate and methylation site specificity Homo sapiens S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4 Drosophila melanogaster S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4 Homo sapiens S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4, SET8 Homo sapiens S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
2.1.1.362 S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4, SET8 Drosophila elegans S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.362 monomer 1 * 45000, SDS-PAGE Homo sapiens
2.1.1.362 monomer 1 * 45000, SDS-PAGE Drosophila elegans