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Literature summary extracted from

  • Thapar, N.; Clarke, S.
    Expression, Purification, and Characterization of the Protein Repair L-Isoaspartyl Methyltransferase from Arabidopsis thaliana (2000), Protein Expr. Purif., 20, 237-251.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.77
-
 Homo sapiens
2.1.1.77
-
 Rattus norvegicus
2.1.1.77
-
 Caenorhabditis elegans
2.1.1.77
-
 Thermotoga maritima
2.1.1.77 cDNA isolation Triticum aestivum
2.1.1.77 cDNA isolation, pcm gene cloned and expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.77 0.00029
-
 VYP (isoAsp) HA
-
 Homo sapiens
2.1.1.77 0.00052
-
 KASA (isoD) LAKY
-
 Homo sapiens
2.1.1.77 0.0019
-
 S-adenosyl-L-methionine
-
 Thermotoga maritima
2.1.1.77 0.0022
-
 S-adenosyl-L-methionine
-
 Homo sapiens
2.1.1.77 0.0028
-
 KASA (isoAsp) LAKY
-
 Thermotoga maritima
2.1.1.77 0.0031
-
 S-adenosyl-L-methionine
-
 Caenorhabditis elegans
2.1.1.77 0.0041
-
 VYP (isoAsp) HA
-
 Thermotoga maritima
2.1.1.77 0.006
-
 S-adenosyl-L-methionine
-
 Arabidopsis thaliana
2.1.1.77 0.00912
-
 KASA (isoAsp) LAKY
-
 Caenorhabditis elegans
2.1.1.77 0.0118
-
 VYP (isoAsp) HA
-
 Escherichia coli
2.1.1.77 0.0127
-
 KASA (isoAsp) LAKY
-
 Triticum aestivum
2.1.1.77 0.0194
-
 VYP (isoD) HA
-
 Caenorhabditis elegans
2.1.1.77 0.03
-
 ovalbumin
-
 Homo sapiens
2.1.1.77 0.043
-
 VYP-L-isoAsp-HA
-
 Zea mays
2.1.1.77 0.0506
-
 KASA (isoAsp) LAKY
-
 Escherichia coli
2.1.1.77 0.0517
-
 VYP-L-isoAsp-HA
-
 Triticum aestivum
2.1.1.77 0.08
-
 KASA-L-isoAsp-LAKY
-
 Arabidopsis thaliana
2.1.1.77 0.092
-
 KASA-L-isoAsp-LAKY
-
 Zea mays
2.1.1.77 0.31
-
 VYP-L-isoAsp-HA
-
 Arabidopsis thaliana
2.1.1.77 0.326
-
 ovalbumin
-
 Thermotoga maritima
2.1.1.77 0.727
-
 ovalbumin
-
 Escherichia coli
2.1.1.77 1.2
-
 ovalbumin native enzyme Arabidopsis thaliana
2.1.1.77 2.66
-
 ovalbumin
-
 Zea mays
2.1.1.77 4.95
-
 ovalbumin
-
 Arabidopsis thaliana
2.1.1.77 5
-
 ovalbumin
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.77 cytoplasm
-
 Homo sapiens 5737
-
2.1.1.77 cytoplasm
-
 Xenopus laevis 5737
-
2.1.1.77 cytosol
-
 Escherichia coli 5829
-
2.1.1.77 cytosol
-
 Homo sapiens 5829
-
2.1.1.77 cytosol
-
 Rattus norvegicus 5829
-
2.1.1.77 cytosol
-
 Arabidopsis thaliana 5829
-
2.1.1.77 cytosol
-
 Caenorhabditis elegans 5829
-
2.1.1.77 cytosol
-
 Thermotoga maritima 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.77 24600
-
 amino acid sequence Arabidopsis thaliana
2.1.1.77 28000
-
 SDS-PAGE Homo sapiens
2.1.1.77 28000
-
 SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Drosophila melanogaster
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Escherichia coli
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Homo sapiens
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Rattus norvegicus
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Triticum aestivum
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Zea mays
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Xenopus laevis
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Caenorhabditis elegans
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Solanum lycopersicum
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Oryza sativa
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Thermotoga maritima
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Arabidopsis thaliana repair enzyme, methylates abnormal L-isoaspartate residues in proteins which arise spontaneously as a result of aging S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.77 Arabidopsis thaliana
-
 Thale cress
-
2.1.1.77 Caenorhabditis elegans
-
-
-
2.1.1.77 Drosophila melanogaster
-
-
-
2.1.1.77 Escherichia coli
-
-
-
2.1.1.77 Homo sapiens
-
 human
-
2.1.1.77 Oryza sativa
-
 rice
-
2.1.1.77 Rattus norvegicus
-
-
-
2.1.1.77 Solanum lycopersicum
-
 tomato, Mill.
-
2.1.1.77 Thermotoga maritima
-
-
-
2.1.1.77 Triticum aestivum
-
 wheat
-
2.1.1.77 Xenopus laevis
-
-
-
2.1.1.77 Zea mays
-
 maize, corn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.77
-
 Triticum aestivum
2.1.1.77 recombinant protein Homo sapiens
2.1.1.77 recombinant protein Rattus norvegicus
2.1.1.77 recombinant protein Arabidopsis thaliana
2.1.1.77 recombinant protein Caenorhabditis elegans
2.1.1.77 recombinant protein Thermotoga maritima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.77 blood
-
 Homo sapiens
-
2.1.1.77 blood
-
 Rattus norvegicus
-
2.1.1.77 brain
-
 Homo sapiens
-
2.1.1.77 brain
-
 Rattus norvegicus
-
2.1.1.77 embryo
-
 Arabidopsis thaliana
-
2.1.1.77 erythrocyte
-
 Homo sapiens
-
2.1.1.77 erythrocyte
-
 Rattus norvegicus
-
2.1.1.77 germ
-
 Triticum aestivum
-
2.1.1.77 leaf
-
 Triticum aestivum
-
2.1.1.77 leaf
-
 Zea mays
-
2.1.1.77 leaf
-
 Arabidopsis thaliana
-
2.1.1.77 leaf
-
 Solanum lycopersicum
-
2.1.1.77 liver
-
 Rattus norvegicus
-
2.1.1.77 oocyte
-
 Xenopus laevis
-
2.1.1.77 seed
-
 Triticum aestivum
-
2.1.1.77 seed
-
 Zea mays
-
2.1.1.77 seed
-
 Arabidopsis thaliana
-
2.1.1.77 seed
-
 Solanum lycopersicum
-
2.1.1.77 seed
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.77 0.0000127
-
 VYP-L-isoAsp-GHG as substrate Arabidopsis thaliana
2.1.1.77 0.00013
-
 gamma-globulin as substrate Arabidopsis thaliana
2.1.1.77 0.000229
-
 VYP-L-isoAsp-RR as substrate Arabidopsis thaliana
2.1.1.77 0.000433
-
 ovalbumin as substrate Arabidopsis thaliana
2.1.1.77 0.000439
-
-
 Arabidopsis thaliana
2.1.1.77 0.001441
-
 VYP-L-isoAsp-CA as substrate Arabidopsis thaliana
2.1.1.77 0.004179
-
 KASA-L-isoAsp-LAKY as substrate Arabidopsis thaliana
2.1.1.77 0.005028
-
 VYP-L-isoAsp-HA as substrate Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.77 S-adenosyl-L-methionine + gamma-globulin
-
 Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + gamma-globulin
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY
-
 Escherichia coli S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY
-
 Thermotoga maritima S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
 Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
 Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
 Zea mays S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
 Oryza sativa S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Escherichia coli S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Zea mays S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Oryza sativa S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Thermotoga maritima S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein D-aspartate
-
 Escherichia coli S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein D-aspartate
-
 Homo sapiens S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein D-aspartate
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Escherichia coli S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Homo sapiens S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Rattus norvegicus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Triticum aestivum S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Zea mays S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Xenopus laevis S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Oryza sativa S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Thermotoga maritima S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate repair enzyme, methylates abnormal L-isoaspartate residues in proteins which arise spontaneously as a result of aging Arabidopsis thaliana S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-CA
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-GHG
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
 Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
 Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
 Zea mays S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
 Oryza sativa S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP-L-isoAsp-RR
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.77 monomer
-
 Escherichia coli
2.1.1.77 monomer
-
 Homo sapiens
2.1.1.77 monomer
-
 Thermotoga maritima
2.1.1.77 monomer 1 * 28000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.1.1.77 protein repair L-isoaspartyl methyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.77 45
-
-
 Escherichia coli
2.1.1.77 45
-
 recombinant enzyme, expressed in E. coli Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.77 55
-
 loses activity rapidly at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.77 7 8 recombinant enzyme, expressed in Escherichia coli Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.77 3 10
-
 Arabidopsis thaliana