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Literature summary extracted from

  • Okamura-Ikeda, K.; Fujiwara, K.; Motokawa, Y.
    Purification and characterization of chicken liver T-protein, a component of the glycine cleavage system (1982), J. Biol. Chem., 257, 135-139.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.2.10 lability of enzyme under the conditions of its purifications and storage Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.10 0.0037
-
 S-aminomethyldihydrolipoylprotein
-
 Gallus gallus
2.1.2.10 0.17
-
 tetrahydrofolate
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.10 mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.10 37000 38000 sedimentation equilibrium centrifugation, gel filtration Gallus gallus
2.1.2.10 41000
-
 1 * 41000, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate Gallus gallus part of the glycine cleavage system dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.10 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.10 641fold purification Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.10 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.10 additional information
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.1.2.10 -20°C, 20 mM Tris-HCl buffer, pH 8.0, 10 mM 2-mercaptoethanol, 50% v/v glycerol, at least 2 months, stable Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.10 additional information component of the glycine cleavage system, T-protein is associated with H-protein forming a complex which is composed of one molecule of each of them Gallus gallus ?
-
 ?
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate decarboxylated glycine moiety attached to H-protein + tetrahydrofolate as substrates Gallus gallus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 r
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate strictly dependent on tetrahydrofolate Gallus gallus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 r
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate part of the glycine cleavage system Gallus gallus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.10 monomer 1 * 41000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.10 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.10 additional information
-
 pI: 9.8 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.10 tetrahydrofolate strictly dependent on tetrahydrofolate Gallus gallus