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Literature summary extracted from

  • Jones, C.E.; Brook, J.M.; Buck, D.; Abell, C.; Smith, A.G.
    Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme (1993), J. Bacteriol., 175, 2125-2130.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.11 panB gene encoding enzyme is cloned, sequenced and 50fold overexpressed in Escherichia coli panB mutant Hfr3000 YA139 containing plasmid pCEJ01, gene is 792 bp long and encodes a protein of 264 amino acids, gene is likely to be cotranscribed with at least one other gene, panB, panC and panD genes are closely clustered at 3.1 min of the Escherichia coli K12 genetic map Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.11 additional information panB mutant Hfr3000 YA139 completely lacks KPHMT Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.11 0.15
-
ketopantoate
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.11 28179
-
6 * 28179, calculated from the amino acid sequence and electrospray mass spectrometry, 6 * 29000, recombinant enzyme expressed in Escherichia coli Hfr3000 YA139/pCEJ01, SDS-PAGE Escherichia coli
2.1.2.11 29000
-
6 * 28179, calculated from the amino acid sequence and electrospray mass spectrometry, 6 * 29000, recombinant enzyme expressed in Escherichia coli Hfr3000 YA139/pCEJ01, SDS-PAGE Escherichia coli
2.1.2.11 174000
-
recombinant enzyme, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Escherichia coli first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.11 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.1.2.11 no modification
-
Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.11 19.3fold purification of recombinant enzyme 50fold overexpressed in E. coli panB mutant Hfr3000 YA139/pCEJ01 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.11 0.007
-
strain K12 Escherichia coli
2.1.2.11 0.162
-
strain Hfr3000 YA139/pCEJ02 Escherichia coli
2.1.2.11 0.348
-
strain Hfr3000 YA139/pCEJ01 Escherichia coli
2.1.2.11 0.666
-
strain Hfr3000 YA139/pSAL38 Escherichia coli
2.1.2.11 3890
-
purified recombinant enzyme from strain Hfr3000 YA139/pCEJ01 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Escherichia coli tetrahydrofolate + 2-dehydropantoate
-
r
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Escherichia coli tetrahydrofolate + 2-dehydropantoate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.11 hexamer 6 * 28179, calculated from the amino acid sequence and electrospray mass spectrometry, 6 * 29000, recombinant enzyme expressed in Escherichia coli Hfr3000 YA139/pCEJ01, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.11 5,10-methylenetetrahydrofolate
-
Escherichia coli
2.1.2.11 tetrahydrofolate
-
Escherichia coli