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Literature summary extracted from

  • Young, M.; Sammons, R.D.; Mueller, W.T.; Benkovic, S.J.
    An antibody probe to determine the native species of glycinamide ribonucleotide transformylase in chicken liver (1984), Biochemistry, 23, 3979-3986.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 55000
-
2 * 55000, SDS-PAGE, sucrose density ultracentrifugation, proteolytic cleavage Gallus gallus
2.1.2.2 110000
-
SDS-PAGE Gallus gallus
2.1.2.2 117000
-
ultracentrifugation Gallus gallus
2.1.2.2 140000
-
sucrose density ultracentrifugation Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Mus musculus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Gallus gallus
-
chicken
-
2.1.2.2 Mus musculus
-
mouse
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2
-
Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.2 L-1210 cell murine leukemia Mus musculus
-
2.1.2.2 liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.2 14.8
-
-
Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 (6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Gallus gallus (6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
2.1.2.2 additional information GAR transformylase activity copurifies with 5,10-methylene-, 5,10-methenyl, and 10-formyl-H4folate sythetase (combined) trifunctional enzyme, EC 6.3.4.3, EC 1.5.1.5 and EC 3.5.4.9 Gallus gallus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.2 dimer homodimer Gallus gallus
2.1.2.2 dimer 2 * 55000, SDS-PAGE, sucrose density ultracentrifugation, proteolytic cleavage Gallus gallus
2.1.2.2 monomer
-
Mus musculus
2.1.2.2 monomer 1 * 117000, ultracentrifugation Gallus gallus