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Literature summary extracted from

  • Beaudet, R.; Mackenzie, R.
    Kinetic mechanism of formininotransferase from porcine liver (1975), Biochim. Biophys. Acta, 410, 252-261.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.5 citrate
-
 Sus scrofa
2.1.2.5 folic acid
-
 Sus scrofa
2.1.2.5 imidazole
-
 Sus scrofa
2.1.2.5 L-glutamate product inhibition Sus scrofa
2.1.2.5 Maleate
-
 Sus scrofa
2.1.2.5 N-formiminoaspartate
-
 Sus scrofa
2.1.2.5 Tris
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.5 0.1
-
 tetrahydrofolate
-
 Sus scrofa
2.1.2.5 2
-
 N-formimino-L-glutamate tetrahydropteroic acid as substrate Sus scrofa
2.1.2.5 2.5
-
 tetrahydropteroic acid
-
 Sus scrofa
2.1.2.5 12
-
 N-formimino-L-glutamate tetrahydrofolate as substrate Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Sus scrofa histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.5 Sus scrofa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.5 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.5 43 45
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.5 additional information molecular enzyme complex with formiminotransferase and cyclodeaminase activity, EC 2.1.2.5 and EC 4.3.1.4, both activities are contained in a single polypeptide chain, no substrate: formiminoaspartate Sus scrofa ?
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Sus scrofa 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate tetrahydropteroic acid can replace tetrahydrofolate Sus scrofa 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydropteroic acid + N-formimino-L-glutamate tetrahydropteroic acid is a good alternate substrate Sus scrofa 5-formiminotetrahydropteroic acid + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.5 homooctamer
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.5 37
-
 assay at Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.5 additional information
-
 additional information 26000 per min per mol or 3250 per min per chain Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.5 tetrahydrofolate folate-dependent enzyme Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.5 0.1
-
 folic acid
-
 Sus scrofa
2.1.2.5 0.9
-
 L-glutamate
-
 Sus scrofa
2.1.2.5 4
-
 Tris
-
 Sus scrofa
2.1.2.5 6
-
 imidazole
-
 Sus scrofa
2.1.2.5 10
-
 formiminoaspartate
-
 Sus scrofa
2.1.2.5 30
-
 Maleate
-
 Sus scrofa
2.1.2.5 60
-
 citrate
-
 Sus scrofa