Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, M.H.; Gautam-Basak, M.; Wooley, C.; Sander, E.G.
    Deoxycytidylate hydroxymethylase: purification, properties, and the role of a thiol group in catalysis (1988), Biochemistry, 27, 1367-1373.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.2.8 dithiothreitol low specific activity samples can be activated by a 30 min room temperature incubation in 50 mM DTT Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.8 5,5'-dithiobis(2-nitrobenzoic acid) complete loss of activity, second-order kinetics, inactivation is reversed by dithiothreitol, inactivation involves the modification of one thiol group per mol of dimeric enzyme Tequatrovirus T4
2.1.2.8 aminopterin competitive inhibitor with respect to tetrahydrofolate Tequatrovirus T4
2.1.2.8 dihydrofolate competitive inhibitor with respect to tetrahydrofolate Tequatrovirus T4
2.1.2.8 methotrexate competitive inhibitor with respect to tetrahydrofolate Tequatrovirus T4
2.1.2.8 N-ethylmaleimide complete loss of activity, second-order kinetics, pH-dependent inactivation, dCMP protects against inactivation, dCMP plus either methotrexate or aminopterin greatly enhances protection, inactivation involves the modification of one thiol group per mol of dimeric enzyme Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.8 0.04
-
 tetrahydrofolate
-
 Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.8 28000
-
 2 * 28000, two equally sized subunits are arranged asymmetrically, SDS-PAGE Tequatrovirus T4
2.1.2.8 51400
-
 gel filtration Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4
-
 Escherichia coli strains Cr 63 and B infected with bacteriophage T4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.8 1026fold purification Tequatrovirus T4
2.1.2.8 Escherichia coli B infected with Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.8 additional information
-
-
 Tequatrovirus T4

Storage Stability

EC Number Storage Stability Organism
2.1.2.8 frozen, 20 mM KH2PO4, pH 6.8, 6 months Tequatrovirus T4
2.1.2.8 room temperature, 24 h, stable Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate stoichiometry of dCMP binding to the dimeric enzyme is 1:1 and depends upon a reduced thiol group, one thiol-containing active site per mol of dimeric enzyme, sequential order of substrate binding Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.8 dimer 2 * 28000, two equally sized subunits are arranged asymmetrically, SDS-PAGE Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.8 30
-
 assay at Tequatrovirus T4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.8 20
-
 room temperature, 24 h, stable Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.8 7.6
-
 assay at Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methylenetetrahydrofolate active cofactor is likely 5,10-methylenetetrahydrofolate Tequatrovirus T4
2.1.2.8 tetrahydrofolate
-
 Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.8 0.001
-
 aminopterin
-
 Tequatrovirus T4
2.1.2.8 0.016
-
 methotrexate
-
 Tequatrovirus T4
2.1.2.8 0.021
-
 dihydrofolate
-
 Tequatrovirus T4