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Literature summary extracted from

  • Graves, K.L.; Hardy, L.W.
    Kinetic and equilibrium alpha-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4 (1994), Biochemistry, 33, 13049-13056.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.8 structural gene 42 encodes enzyme Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.8 D179A mutant not inactivated by 5-fluoro-dUMP Tequatrovirus T4
2.1.2.8 D179N mutant with altered substrate preference, favoring dUMP rather than dCMP Tequatrovirus T4
2.1.2.8 D179N mutation of active site, 10fold increased rate of inhibition by 5-fluoro-dUMP Tequatrovirus T4
2.1.2.8 D179S mutant not inactivated by 5-fluoro-dUMP Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.8 5-fluorodeoxyuridylate inactivates by formation of a covalent complex containing enzyme, inhibitor and methylenetetrahydrofolate in a time-dependent manner, 1 mol 5-fluoro-dUMP per enzyme monomer, no substantial inhibition of wild-type enzyme in vivo, inhibition mechanism Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.8 28600
-
 x * 28600 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4
-
 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4
-
 Escherichia coli JM101 infected with bacteriophage T4
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate mechanism Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
 Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Cys-148 is catalytically essential Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.8 ? x * 28600 Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methylenetetrahydrofolate
-
 Tequatrovirus T4
2.1.2.8 tetrahydrofolate
-
 Tequatrovirus T4