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Literature summary extracted from

  • Lee, C.P.; Seong, B.L.; RajBhandary, U.L.
    Structural and sequence elements important for recognition of Escherichia coli formylmethionine tRNA by methionyl-tRNA transformylase are clustered in the acceptor stem (1991), J. Biol. Chem., 266, 18012-18017.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Escherichia coli
2.1.2.9 0.0017
-
 L-methionyl-tRNAfMet
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Escherichia coli
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity determinants at the end of the acceptor stem of tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O nucleotide-mutants of tRNAfMet as substrates Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 37
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.9 10-formyltetrahydrofolate
-
 Escherichia coli
2.1.2.9 tetrahydrofolate
-
 Escherichia coli