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Literature summary extracted from

  • Samuel, C.E.; Rabinowitz, J.C.
    Initiation of protein synthesis by folate-sufficient and folate-deficient Streptococcus faecalis R: Partial purification and properties of methionyl-transfer ribonucleic acid synthetase and methionyl-transfer ribonucleic acid formyltransferase (1974), J. Bacteriol., 118, 21-31.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.2.9 additional information not stimulated by ATP or GTP Escherichia coli
2.1.2.9 additional information not stimulated by ATP or GTP Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.9 additional information not inhibited by folic acid; not inhibited by pteroic acid, aminopterin, methionyl-tRNAmMet and uncharged tRNAmMet Enterococcus faecalis
2.1.2.9 additional information E. coli A19 enzyme is not inhibited by uncharged tRNAmMet and tRNAfMet from E. coli K12 or Streptococcus faecalis Escherichia coli
2.1.2.9 tetrahydrofolate competitive inhibition Enterococcus faecalis
2.1.2.9 tRNAfMet tRNAfMet from Streptococcus faecalis or E. coli K12, strong inhibition Enterococcus faecalis
2.1.2.9 tRNAfMet non-aminoacylated tRNAfMet is a competitive inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 0.00072 0.0009 L-methionyl-tRNAfMet
-
 Enterococcus faecalis
2.1.2.9 0.006 0.007 10-formyltetrahydrofolate
-
 Enterococcus faecalis
2.1.2.9 0.011 0.016 10-formyltetrahydropteroyltriglutamate
-
 Enterococcus faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 cytoplasm
-
 Escherichia coli 5737
-
2.1.2.9 cytoplasm
-
 Enterococcus faecalis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.9 Mg2+ requirement Escherichia coli
2.1.2.9 Mg2+ requirement Enterococcus faecalis
2.1.2.9 Mg2+ optimal concentration: 30 mM Escherichia coli
2.1.2.9 Mg2+ optimal concentration: 30 mM Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.9 29900
-
 sucrose density gradient centrifugation Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Enterococcus faecalis involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Enterococcus faecalis R / ATCC 8043 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli A19 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Enterococcus faecalis
-
-
-
2.1.2.9 Enterococcus faecalis R / ATCC 8043
-
-
-
2.1.2.9 Escherichia coli
-
-
-
2.1.2.9 Escherichia coli A19
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9
-
 Escherichia coli
2.1.2.9 100-300fold, partial purification Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.9 0.53
-
-
 Escherichia coli
2.1.2.9 0.64 0.73
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor Enterococcus faecalis R / ATCC 8043 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Enterococcus faecalis R / ATCC 8043 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Enterococcus faecalis R / ATCC 8043 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli A19 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli A19 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydropteroyltriglutamate + L-methionyl-tRNAfMet + H2O 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can act as donor Enterococcus faecalis tetrahydropteroyltriglutamate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Escherichia coli ?
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Enterococcus faecalis ?
-
 ?
2.1.2.9 additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Escherichia coli ?
-
 ?
2.1.2.9 additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Enterococcus faecalis ?
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Enterococcus faecalis R / ATCC 8043 ?
-
 ?
2.1.2.9 additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Enterococcus faecalis R / ATCC 8043 ?
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Escherichia coli A19 ?
-
 ?
2.1.2.9 additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Escherichia coli A19 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 30
-
 assay at Escherichia coli
2.1.2.9 30
-
 assay at Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.9 7
-
 about Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.9 10-formyltetrahydrofolate
-
 Escherichia coli
2.1.2.9 10-formyltetrahydrofolate
-
 Enterococcus faecalis
2.1.2.9 additional information no cofactors: ribonucleotide mono- or triphosphates Escherichia coli
2.1.2.9 additional information no cofactors: ribonucleotide mono- or triphosphates Enterococcus faecalis
2.1.2.9 tetrahydrofolate
-
 Escherichia coli
2.1.2.9 tetrahydrofolate
-
 Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.9 0.00018 0.00057 tRNAfMet
-
 Enterococcus faecalis
2.1.2.9 0.024
-
 tetrahydrofolate
-
 Enterococcus faecalis