BRENDA - Enzyme Database show

Purification and characterization of acetylcoenzyme A: deacetylvindoline 4-O-acetyltransferase from Catharanthus roseus

Power, R.; Kurz, W.G.W.; de Luca, V.; Arch. Biochem. Biophys. 279, 370-376 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.107
CoA
0.037 mM, 50% inhibition
Catharanthus roseus
2.3.1.107
secologanin
0.5 mM, 25% inhibition
Catharanthus roseus
2.3.1.107
tabersonine
0.045 mM, 50% inhibition
Catharanthus roseus
2.3.1.107
tryptamine
0.5 mM, 28% inhibition
Catharanthus roseus
2.3.1.107
vindoline
0.5 mM, 40% inhibition
Catharanthus roseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.107
0.0013
-
deacetylvindoline
-
Catharanthus roseus
2.3.1.107
0.0065
-
acetyl-CoA
-
Catharanthus roseus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.107
acetyl-CoA + 17-O-deacetylvindoline
Catharanthus roseus
the enzyme catalyzes the final step in the biosynthesis of vindoline
CoA + vindoline
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.107
Catharanthus roseus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.107
-
Catharanthus roseus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.107
leaf
-
Catharanthus roseus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.107
additional information
-
-
Catharanthus roseus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.107
acetyl-CoA + 17-O-deacetylvindoline
-
486108
Catharanthus roseus
CoA + vindoline
-
-
-
?
2.3.1.107
acetyl-CoA + 17-O-deacetylvindoline
the enzyme catalyzes the final step in the biosynthesis of vindoline
486108
Catharanthus roseus
CoA + vindoline
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.107
7.5
9
-
Catharanthus roseus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.107
5.5
11
activity increases from pH 5.5 to 7 and decreases rapidly between pH 9 and 11
Catharanthus roseus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.107
CoA
0.037 mM, 50% inhibition
Catharanthus roseus
2.3.1.107
secologanin
0.5 mM, 25% inhibition
Catharanthus roseus
2.3.1.107
tabersonine
0.045 mM, 50% inhibition
Catharanthus roseus
2.3.1.107
tryptamine
0.5 mM, 28% inhibition
Catharanthus roseus
2.3.1.107
vindoline
0.5 mM, 40% inhibition
Catharanthus roseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.107
0.0013
-
deacetylvindoline
-
Catharanthus roseus
2.3.1.107
0.0065
-
acetyl-CoA
-
Catharanthus roseus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.107
acetyl-CoA + 17-O-deacetylvindoline
Catharanthus roseus
the enzyme catalyzes the final step in the biosynthesis of vindoline
CoA + vindoline
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.107
-
Catharanthus roseus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.107
leaf
-
Catharanthus roseus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.107
additional information
-
-
Catharanthus roseus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.107
acetyl-CoA + 17-O-deacetylvindoline
-
486108
Catharanthus roseus
CoA + vindoline
-
-
-
?
2.3.1.107
acetyl-CoA + 17-O-deacetylvindoline
the enzyme catalyzes the final step in the biosynthesis of vindoline
486108
Catharanthus roseus
CoA + vindoline
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.107
7.5
9
-
Catharanthus roseus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.107
5.5
11
activity increases from pH 5.5 to 7 and decreases rapidly between pH 9 and 11
Catharanthus roseus