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Literature summary extracted from

  • Yamashita, S.; Numa, S.
    Glycerophosphate acyltransferase from rat liver (1981), Methods Enzymol., 71, 550-554.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 phosphatidylcholine
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 5,5'-dithiobis(2-nitro-benzoate)
-
 Rattus norvegicus
2.3.1.15 acyl-CoA strong substrate inhibition Rattus norvegicus
2.3.1.15 N-ethylmaleimide
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.2
-
 sn-glycerol 3-phosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 Ca2+
-
 Rattus norvegicus
2.3.1.15 Co2+ at high concentrations Rattus norvegicus
2.3.1.15 Mg2+ activation, slightly less than Ca2+ Rattus norvegicus
2.3.1.15 Mn2+ less effective than Ca2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Rattus norvegicus
-
 male
-
2.3.1.15 Rattus norvegicus
-
 Wistar strain
-
2.3.1.15 Rattus norvegicus Wistar
-
 Wistar strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.0022
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Rattus norvegicus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Rattus norvegicus Wistar CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 arachidonoyl-CoA + sn-glycerol 3-phosphate poor substrate Rattus norvegicus CoA + 1-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 linoleoyl-CoA + sn-glycerol 3-phosphate poor substrate Rattus norvegicus CoA + 1-linoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 linoleoyl-CoA + sn-glycerol 3-phosphate poor substrate Rattus norvegicus Wistar CoA + 1-linoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate poor substrate Rattus norvegicus CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate poor substrate Rattus norvegicus Wistar CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate best acyl donor Rattus norvegicus CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate best acyl donor Rattus norvegicus Wistar CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus Wistar CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 6.6 9 both microsomal and mitochondrial enzymes exhibit a broad optimum Rattus norvegicus