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Literature summary extracted from

  • Mishra, S.; Kamisaka, Y.
    Purification and characterization of thiol-reagent-sensitive glycerol-3-phosphate acyltransferase from the membrane fraction of an oleaginous fungus (2001), Biochem. J., 355, 315-322.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 dioleoyl phosphatidic acid very strong activation Umbelopsis ramanniana
2.3.1.15 dioleoyl phosphatidylserine best phospholipid for activation Umbelopsis ramanniana
2.3.1.15 dithiothreitol 1 mM, 82% activation Umbelopsis ramanniana
2.3.1.15 Phospholipids required for activity Umbelopsis ramanniana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 5,5'-dithiobis(2-nitro-benzoate) 1 mM, 77% inhibition Umbelopsis ramanniana
2.3.1.15 Ca2+ 5 mM, 70% inhibition Umbelopsis ramanniana
2.3.1.15 EDTA 1 mM, 79% inhibition Umbelopsis ramanniana
2.3.1.15 Mg2+ 5 mM, 75% inhibition Umbelopsis ramanniana
2.3.1.15 N-ethylmaleimide 5 mM, 79% inhibition Umbelopsis ramanniana
2.3.1.15 p-chloromercuribenzoate 1 mM, 64% inhibition Umbelopsis ramanniana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.015
-
 oleoyl-CoA
-
 Umbelopsis ramanniana
2.3.1.15 0.025
-
 glycerol 3-phosphate
-
 Umbelopsis ramanniana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 membrane
-
 Umbelopsis ramanniana 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 Mn2+ 2.5 mM, 53% activation, 10 mM, 100% activation Umbelopsis ramanniana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 45000
-
 x * 45000, SS-PAGE Umbelopsis ramanniana

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Umbelopsis ramanniana
-
 var. angulispora
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 heparin-Sepharose, Yellow 86-Sepharose, Superdex 200, hydroxylapatite Umbelopsis ramanniana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 1.45
-
-
 Umbelopsis ramanniana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Umbelopsis ramanniana CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 myristoyl-CoA + sn-glycerol 3-phosphate 20% of activity with oleoyl-CoA Umbelopsis ramanniana CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 n-dodecanoyl-CoA + sn-glycerol 3-phosphate very low activity Umbelopsis ramanniana CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 octanoyl-CoA + sn-glycerol 3-phosphate very low activity Umbelopsis ramanniana CoA + 1-n-octanoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate best acyl-donor Umbelopsis ramanniana CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate 20% of activity with oleoyl-CoA Umbelopsis ramanniana CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate 20% of activity with oleoyl-CoA Umbelopsis ramanniana CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 ? x * 45000, SS-PAGE Umbelopsis ramanniana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7
-
-
 Umbelopsis ramanniana