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Literature summary extracted from

  • Coleman, R.A.; Bell, R.M.
    Triacylglycerol synthesis in isolated fat cells. Studies on the microsomal diacylglycerol acyltransferase activity using ethanol-dispersed diacylglycerols (1976), J. Biol. Chem., 251, 4537-4543.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 bovine serum albumin activation, 1.0-1.5 mg/ml Rattus norvegicus
2.3.1.20 ethanol highest activity when diacylglycerol is in ethanol Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 1,2-diacyl-sn-glycerol dicaprin, dimyristin, dipalmitin, high concentration Rattus norvegicus
2.3.1.20 1-Hexanol
-
 Rattus norvegicus
2.3.1.20 2-butanol
-
 Rattus norvegicus
2.3.1.20 acyl-CoA C-10-CoA to C-16-CoA, high concentration Rattus norvegicus
2.3.1.20 dioxane
-
 Rattus norvegicus
2.3.1.20 Gum arabic
-
 Rattus norvegicus
2.3.1.20 MnCl2 2 mM Rattus norvegicus
2.3.1.20 Tween 20
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Rattus norvegicus
-
-
2.3.1.20 additional information subcellular distribution Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+ omission results in 50% activity Rattus norvegicus
2.3.1.20 Mg2+ 2-10 mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol Rattus norvegicus last reaction in triacylglycerol synthesis CoA + triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Rattus norvegicus
-
 female Charles River CD
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 adipocyte
-
 Rattus norvegicus
-
2.3.1.20 intestinal mucosa
-
 Rattus norvegicus
-
2.3.1.20 liver
-
 Rattus norvegicus
-
2.3.1.20 additional information distribution, overview Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.20 -15°C, several months, microsomal preparation Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacylglycerol last reaction in triacylglycerol synthesis Rattus norvegicus CoA + triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-dioleoylglycerol
-
 Rattus norvegicus CoA + 3-acyl-1,2-dioleoylglycerol
-
 ?
2.3.1.20 arachidonoyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + 3-arachidonoyl-1,2-diacylglycerol
-
 ?
2.3.1.20 decanoyl-CoA + 1,2-diacylglycerol saturated fatty acyl-CoAs from C-8 to C-18 with decanoyl-CoA as the best Rattus norvegicus CoA + 1,2-diacyl-3-decanoylglycerol
-
 ?
2.3.1.20 linoleoyl-CoA + 1,2-diacylglycerol poor donor Rattus norvegicus CoA + 1,2-diacyl-3-linoleoylglycerol
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-dioleoyl-sn-glycerol i.e. diolein, at 34% the rate of the acylation with stearoyl-CoA Rattus norvegicus CoA + trioleoylglycerol
-
 ?
2.3.1.20 palmitoyl-CoA + 1,2-diacylglycerol
-
 Rattus norvegicus CoA + 1,2-diacyl-3-palmitoylglycerol
-
 ?
2.3.1.20 palmitoyl-CoA + didecanoylglycerol i.e. dicaprin Rattus norvegicus CoA + 1,2-didecanoyl-3-palmitoylglycerol
-
 ?
2.3.1.20 palmitoyl-CoA + dihexanoylglycerol i.e. dicaproin Rattus norvegicus CoA + dihexanoyl-palmitoylglycerol
-
 ?
2.3.1.20 stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Rattus norvegicus CoA + 1,2-dioleoyl-3-stearoylglycerol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 additional information
-
 assay at various temperatures from 24°C to 40°C Rattus norvegicus
2.3.1.20 23
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.20 23
-
 at least 15 min stable Rattus norvegicus
2.3.1.20 28
-
 stable below, about 30% loss of activity after 15 min Rattus norvegicus
2.3.1.20 37
-
 t1/2: 5 min Rattus norvegicus
2.3.1.20 42
-
 t1/2: 1.5 min Rattus norvegicus
2.3.1.20 48
-
 t1/2: 1 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.5 8
-
 Rattus norvegicus