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Literature summary extracted from

  • Nolte, G.; Sussmuth, R.
    Purification and characterization of chloramphenicol acetyltransferase from Flavobacterium CB60 (1987), J. Gen. Microbiol., 133, 2115-2122.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.28 loss of activity by freezing and thawing can be prevented by adding 10% glycerol Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.28 1,5-Difluoro-2,4-dinitrobenzene
-
 Flavobacterium sp.
2.3.1.28 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Flavobacterium sp.
2.3.1.28 NEM
-
 Flavobacterium sp.
2.3.1.28 PCMB
-
 Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.28 0.03
-
 chloramphenicol
-
 Flavobacterium sp.
2.3.1.28 0.065
-
 acetyl-CoA
-
 Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.28 26600
-
 3 * 26600, SDS-PAGE Flavobacterium sp.
2.3.1.28 80000
-
 gel filtration Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.28 Flavobacterium sp.
-
 CB60
-
2.3.1.28 Flavobacterium sp. CB60
-
 CB60
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.28
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.28 367.7
-
-
 Flavobacterium sp.

Storage Stability

EC Number Storage Stability Organism
2.3.1.28 -20 C, stable for several months Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.28 acetyl-CoA + chloramphenicol
-
 Flavobacterium sp. CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol
-
 Flavobacterium sp. CB60 CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol 1-acetate no activity Flavobacterium sp. CoA + chloramphenicol 1,3-diacetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol 1-acetate no activity Flavobacterium sp. CB60 CoA + chloramphenicol 1,3-diacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.28 trimer 3 * 26600, SDS-PAGE Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.28 60
-
 nearly complete inactivation within 10 min Flavobacterium sp.