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Literature summary extracted from

  • Alberts, A.W.; Majerus, P.W.; Vagelos, P.R.
    beta-Ketoacyl acyl carrier protein synthase (1969), Methods Enzymol., 14, 57-60.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.41 2-mercaptoethanol required in high concentration, 0.5 M Escherichia coli
2.3.1.41 dithiothreitol activation Escherichia coli
2.3.1.41 dithiothreitol can replace 2-mercaptoethanol Escherichia coli
2.3.1.41 EDTA 7 mM, stimulates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 iodoacetamide after incubation with 2-mercaptethanol Escherichia coli
2.3.1.41 N-ethylmaleimide after incubation with 2-mercaptoethanol Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 0.019
-
 acetyl-[acyl-carrier protein] pH 7.0 Escherichia coli
2.3.1.41 0.025
-
 malonyl-[acyl-carrier protein] pH 7.0 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Escherichia coli part of non-associated fatty acid synthase system of plants and prokaryotes 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Escherichia coli involved in biosynthesis of saturated fatty acids 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 partial Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 3.81
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.41 -20°C, several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] part of non-associated fatty acid synthase system of plants and prokaryotes Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] involved in biosynthesis of saturated fatty acids Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.41 7 7.8
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.41 6.5 8 sharp drop in activity below pH 6.5 and above pH 8.0 Escherichia coli