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Literature summary extracted from
- Multiple forms of beta-ketoacyl-acyl carrier protein synthetase in Escherichia coli (1975), J. Biol. Chem., 250, 5289-5294.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.41 | iodoacetamide | acyl-[acyl-carrier-protein]-derivatives, e.g. acetyl-[acyl-carrier-protein], hexanoyl-CoA, octanoyl-CoA, decanoyl-CoA protect; synthase I and II | Escherichia coli |  |
| 2.3.1.179 | iodoacetamide | prior incubation of the enzymes with fatty acyl thioesters prevents inhibition | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.41 | 0.012 | - |
cis-3-decenoyl-[acyl-carrier protein] | synthase I | Escherichia coli | |
| 2.3.1.41 | 0.014 | - |
cis-3-decenoyl-[acyl-carrier protein] | synthase II | Escherichia coli | |
| 2.3.1.41 | 0.017 | - |
cis-9-hexadecenoyl-[acyl-carrier protein] | synthase II | Escherichia coli | |
| 2.3.1.41 | 0.031 | - |
acetyl-[acyl-carrier protein] | synthase I | Escherichia coli | |
| 2.3.1.41 | 0.04 | - |
acetyl-[acyl-carrier protein] | synthase II | Escherichia coli | |
| 2.3.1.41 | 0.138 | - |
cis-9-hexadecenoyl-[acyl-carrier protein] | synthase I | Escherichia coli | |
| 2.3.1.179 | 0.014 | - |
acetyl-[acyl-carrier protein] | pH 7.2 | Escherichia coli | |
| 2.3.1.179 | 0.017 | - |
cis-3-decenoyl-[acyl-carrier protein] | pH 7.2 | Escherichia coli | |
| 2.3.1.179 | 0.04 | - |
palmitoleoyl-[acyl-carrier protein] | pH 7.2 | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 66000 | - |
synthase I, gel filtration | Escherichia coli |
| 2.3.1.41 | 76000 | - |
synthase II, gel filtration | Escherichia coli |
| 2.3.1.179 | 76000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | part of non-associated fatty acid synthase system of plants and prokaryotes | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | involved in biosynthesis of mono-unsaturated long-chain fatty acids | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.41 | Escherichia coli | - |
B | - |
| 2.3.1.41 | Escherichia coli | - |
synthases I and II | - |
| 2.3.1.41 | Escherichia coli B / ATCC 11303 | - |
B | - |
| 2.3.1.179 | Escherichia coli | P0AAI5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.41 | synthases I and II | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 7.5 | - |
purified synthase II | Escherichia coli |
| 2.3.1.41 | 9 | - |
purified synthase I | Escherichia coli |
| 2.3.1.179 | 7.5 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.41 | acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| 2.3.1.41 | acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli B / ATCC 11303 | acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | part of non-associated fatty acid synthase system of plants and prokaryotes | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | involved in biosynthesis of mono-unsaturated long-chain fatty acids | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein | - |
? | |
| 2.3.1.41 | cis-9-hexadecenoyl-CoA + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxo-hexadecenoyl-[acyl-carrier protein] + CO2 + CoA | - |
? | |
| 2.3.1.41 | cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | poor substrate, synthase I | Escherichia coli | 3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| 2.3.1.41 | cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | best substrate, synthase II | Escherichia coli | 3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| 2.3.1.41 | cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | poor substrate, synthase I | Escherichia coli B / ATCC 11303 | 3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| 2.3.1.41 | cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates | Escherichia coli | cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates | Escherichia coli B / ATCC 11303 | cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | additional information | synthase I and II have nearly the same substrate specificity | Escherichia coli | ? | - |
? | |
| 2.3.1.41 | additional information | synthase I and II have nearly the same substrate specificity | Escherichia coli B / ATCC 11303 | ? | - |
? | |
| 2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| 2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Escherichia coli B / ATCC 11303 | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| 2.3.1.179 | acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | acetoacetyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.179 | cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | ? + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.179 | palmitoleoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | cis-vaccenoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.1.179 | beta-ketoacyl-ACP synthase II | - |
Escherichia coli |
| 2.3.1.179 | beta-ketoacyl-acyl carrier protein synthetase II | - |
Escherichia coli |
| 2.3.1.179 | FabF | - |
Escherichia coli |
| 2.3.1.179 | KAS II | - |
Escherichia coli |
| 2.3.1.179 | KASII | - |
Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 43 | - |
t1/2 synthase I: 18 min, t1/2 synthase II: 81 min | Escherichia coli |
| 2.3.1.179 | 43 | - |
t1/2: 81 min | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 5.5 | 6.1 | synthase II | Escherichia coli |
| 2.3.1.41 | 7.2 | - |
synthase I | Escherichia coli |
| 2.3.1.179 | 5.5 | 6.1 | - |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 5.2 | 7.6 | synthase II, about half-maximal activity at pH 5.2 and pH 7.6 | Escherichia coli |
| 2.3.1.41 | 6.3 | 8 | synthase I, about half-maximal activity at pH 6.3 and pH 8.0 | Escherichia coli |
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