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Literature summary extracted from

  • Vandamme-Feldhaus, V.; Schauer, R.
    Characterization of the enzymic 7-O-acetylation of sialic acids and evidence for enzymic O-acetyl migration from C-7 to C-9 in bovine submandibular gland (1998), J. Biochem., 124, 111-121.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.45 2-mercaptoethanol slightly activates Bos taurus
2.3.1.45 acetic acid anhydride activates Bos taurus
2.3.1.45 dithiothreitol slightly activates Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.45 CoA concentration-dependent Bos taurus
2.3.1.45 Cu2+
-
 Bos taurus
2.3.1.45 diethyl dicarbonate inhibits intramolecular migration of the acetyl group from position 7 to 9 Bos taurus
2.3.1.45 methylamine slightly Bos taurus
2.3.1.45 N-Acetylimidazole
-
 Bos taurus
2.3.1.45 N-bromosuccinimide slightly Bos taurus
2.3.1.45 p-chloromercuribenzoate
-
 Bos taurus
2.3.1.45 Triton X-100 above 0.02% Bos taurus
2.3.1.45 Zn2+
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.45 0.00163
-
 acetyl-CoA
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.45 Golgi vesicle
-
 Bos taurus
-
-
2.3.1.45 microsome
-
 Bos taurus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.45 acetyl-CoA + sialic acid Bos taurus
-
 CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.45 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.45 acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-7-O(or 9-O)-acetylneuraminate 7-O-acetyl groups can migrate to the 9-position Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.45 submandibular gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.45 additional information
-
 subcellular distribution Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.45 acetyl-CoA + N-acetylneuraminate glycosidically bound N-acetylneuraminate Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate O-acetylation at position 7, 9-O-acetylated product appears after migration of the acetyl group from position 7 to 9 ?
2.3.1.45 acetyl-CoA + sialic acid
-
 Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?
2.3.1.45 additional information reaction affords the transport of the acetyl group by a translocase across the Golgi membrane, followed by the enzymatic transfer of the acetyl group onto sialic acid in the Golgi lumen Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.45 22
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.45 6.5
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.45 0.0112
-
 CoA
-
 Bos taurus