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Literature summary extracted from

  • Yoshioka, K.; Hashimoto, N.
    Ester formation by alcohol acetyltransferase from brewer's yeast (1981), Agric. Biol. Chem., 45, 2183-2190.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.84 2,4,6-Trinitrobenzenesulfonic acid
-
 Saccharomyces uvarum
2.3.1.84 5,5'-dithiobis(2-nitrobenzoate) 99% inhibition Saccharomyces uvarum
2.3.1.84 Ag+ 1 mM, 92% inhibition Saccharomyces uvarum
2.3.1.84 Cd2+ 1 mM, complete inhibition Saccharomyces uvarum
2.3.1.84 Co2+ 1 mM, 83% inhibition Saccharomyces uvarum
2.3.1.84 Cu2+ 1 mM, 99% inhibition Saccharomyces uvarum
2.3.1.84 ergosterol 2 mM, 73% inhibition Saccharomyces uvarum
2.3.1.84 Hg2+ 1 mM, 98% inhibition Saccharomyces uvarum
2.3.1.84 iodoacetate
-
 Saccharomyces uvarum
2.3.1.84 linoleic acid 2 mM, 97% inhibition Saccharomyces uvarum
2.3.1.84 linolenic acid 2 mM, 97% inhibition Saccharomyces uvarum
2.3.1.84 monoolein 2 mM, 76% inhibition Saccharomyces uvarum
2.3.1.84 monostearin 2 mM, 58% inhibition Saccharomyces uvarum
2.3.1.84 Ni2+ 1 mM, 68% inhibition Saccharomyces uvarum
2.3.1.84 oleoyl-CoA 2 mM, 87% inhibition Saccharomyces uvarum
2.3.1.84 p-chloromercuribenzoate 98% inhibition Saccharomyces uvarum
2.3.1.84 Pb2+ 1 mM, complete inhibition Saccharomyces uvarum
2.3.1.84 phosphatidylinositol 2 mM, 97% inhibition Saccharomyces uvarum
2.3.1.84 phosphatidylserine 2 mM, 88% inhibition Saccharomyces uvarum
2.3.1.84 Tween 60 2 mM, 77% inhibition Saccharomyces uvarum
2.3.1.84 Tween 80 2 mM, 89% inhibition Saccharomyces uvarum
2.3.1.84 Zn2+ 1 mM, 97% inhibition Saccharomyces uvarum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.84 membrane cell membrane bound Saccharomyces uvarum 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.84 220000
-
 gel filtration Saccharomyces uvarum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.84 Saccharomyces uvarum
-
 brewery lager yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.84 Sepharose 6B, DEAE-Sephadex A50, partially purified Saccharomyces uvarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.84 0.00729
-
-
 Saccharomyces uvarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.84 butanol + acetyl-CoA
-
 Saccharomyces uvarum butyl acetate + CoA
-
 ?
2.3.1.84 ethanol + acetyl-CoA
-
 Saccharomyces uvarum ethyl acetate + CoA
-
 ?
2.3.1.84 ethanol + butyryl-CoA
-
 Saccharomyces uvarum ethyl butyrate + CoA
-
 ?
2.3.1.84 hexanol + acetyl-CoA
-
 Saccharomyces uvarum hexyl acetate + CoA
-
 ?
2.3.1.84 isoamylalcohol + acetyl-CoA
-
 Saccharomyces uvarum isoamyl acetate + CoA
-
 ?
2.3.1.84 methanol + acetyl-CoA
-
 Saccharomyces uvarum methyl acetate + CoA
-
 ?
2.3.1.84 pentanol + acetyl-CoA
-
 Saccharomyces uvarum pentyl acetate + CoA
-
 ?
2.3.1.84 propanol + acetyl-CoA low activity Saccharomyces uvarum propyl acetate + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.84 30
-
-
 Saccharomyces uvarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.84 10 40 25% of maximal activity at 10°C, 50% of maximal activity at 40°C Saccharomyces uvarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.84 7 8
-
 Saccharomyces uvarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.84 5 8.5 approx. 40% of maximal activity at pH 5.0, 80% of maximal activity at pH 8.5 Saccharomyces uvarum