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Literature summary extracted from

  • Middleton, B.
    The kinetic mechanism and properties of the cytoplasmic acetoacetyl-coenzyme A thiolase from rat liver (1974), Biochem. J., 139, 109-121.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 CoA competitive vs. acetoacetyl-CoA in thiolysis Rattus norvegicus
2.3.1.9 iodoacetamide 0.005 mM, inactivation half-life: 3 min Rattus norvegicus
2.3.1.9 Mg2+ inhibits the rate of acetoacetyl-CoA thiolysis but not the rate of synthesis of acetoacetyl-CoA Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.9 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 40000
-
 4 * 40000, enzyme A and B, SDS-PAGE Rattus norvegicus
2.3.1.9 170000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Rattus norvegicus
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 pH 5.5, DEAE-cellulose, calcium phosphate column, celulose phosphate column Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.9 2 acetyl-CoA = CoA + acetoacetyl-CoA mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 37
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA thiolysis is strongly preferred Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 40000, enzyme A and B, SDS-PAGE Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.9 0.067
-
 CoA
-
 Rattus norvegicus