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Literature summary extracted from

  • Towler, D.E.; Adams, S.P.; Eubanks, S.R.; Towery, D.S.; Jackson-Machelski, E.; Glaser, L.; Gordon, J.I.
    Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase [published erratum appears in Proc Natl Acad Sci U S A 1987 Nov;84(21):7523] (1987), Proc. Natl. Acad. Sci. USA, 84, 2708-2712.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.97 EDTA slight activation Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 1,10-phenanthroline 0.1 mM, 30% inhibition Saccharomyces cerevisiae
2.3.1.97 diethyldicarbonate
-
 Saccharomyces cerevisiae
2.3.1.97 p-hydroxymercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 additional information
-
 additional information
-
 Saccharomyces cerevisiae
2.3.1.97 0.016
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 0.016
-
 Gly-(diaminobutyrate-gamma-NH2-4-azido-3-iodosalicylamide)-L-Ala-L-Ala-L-Ser-S-Ala-L-Arg-L-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 0.04
-
 Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys
-
 Saccharomyces cerevisiae
2.3.1.97 0.06
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 0.06
-
 Gly-Asn-Phe-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 0.3
-
 Gly-Asn-Ala-Pro-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 0.3
-
 Gly-Ala-Arg-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 1.3
-
 Gly-Asn-Ala-Ala-Ala-Ala
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.97 additional information no requirement for divalent cations Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.97 55000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.97 tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] requires alanine at position 5 of substrate peptides Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + Gly-Ala-Arg-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Ala-Arg-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Ala-Pro-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Pro-Ala-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Phe-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Phe-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide substrate specificity Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide myristoyl-peptides can compete with myristoyl-CoA for the binding site Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide overview peptides and proteins Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide specific for Gly at N-terminus Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.97 monomer 1 * 55000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 NMT
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.97 7.5 8
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.97 7 9 about half-maximal activity above pH 9.0 and below pH 7.0, inactive at pH 6.0 Saccharomyces cerevisiae