Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rhodes, M.J.C.; Wooltorton, L.S.C.
    The enzymic conversion of hydroxycinnamic acids to p-coumarylquinic and chlorogenic acids in tomato fruits (1976), Phytochemistry, 15, 947-951.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.99 bovine serum albumin not Solanum lycopersicum
2.3.1.99 EDTA activation Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.99 HEPES
-
 Solanum lycopersicum
2.3.1.99 additional information no inhibition by bovine serum albumin, Mg2+ or dithioerythritol Solanum lycopersicum
2.3.1.99 N-[2-Acetamido]-2-iminodiacetic acid i.e. N-[carbamoylmethyl]iminodiacetic acid or ADA Solanum lycopersicum
2.3.1.99 Tris/HCl
-
 Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 0.003
-
 coumaroyl-CoA
-
 Solanum lycopersicum
2.3.1.99 0.029
-
 caffeoyl-CoA
-
 Solanum lycopersicum
2.3.1.99 0.06
-
 chlorogenic acid
-
 Solanum lycopersicum
2.3.1.99 0.084
-
 CoA
-
 Solanum lycopersicum
2.3.1.99 0.092
-
 coumaroylquinate
-
 Solanum lycopersicum
2.3.1.99 0.43
-
 quinate with coumaroyl-CoA Solanum lycopersicum
2.3.1.99 0.63
-
 quinate with caffeoyl-CoA Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.99 additional information Solanum lycopersicum enzyme involved in the biosynthesis of chlorogenic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Solanum lycopersicum
-
 var. Eurocross BB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.99
-
 Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.99 fruit
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 additional information
-
-
 Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
2.3.1.99 -20°C, at least 2 months Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 4-coumaroyl-CoA + quinate best substrate Solanum lycopersicum CoA + 5-O-(4-coumaroyl)quinate
-
 r
2.3.1.99 4-coumaroyl-CoA + quinate coumaric acid, caffeic acid cannot replace coumaroyl-CoA Solanum lycopersicum CoA + 5-O-(4-coumaroyl)quinate
-
 r
2.3.1.99 4-coumaroyl-CoA + quinate poor substrates of the reverse reaction are the 3'- or 4'-coumaroylquinates Solanum lycopersicum CoA + 5-O-(4-coumaroyl)quinate
-
 r
2.3.1.99 4-coumaroyl-CoA + quinate not: glucose Solanum lycopersicum CoA + 5-O-(4-coumaroyl)quinate
-
 r
2.3.1.99 caffeoyl-CoA + quinate
-
 Solanum lycopersicum CoA + 5-O-caffeoylquinate i.e. chlorogenic acid r
2.3.1.99 caffeoyl-CoA + quinate
-
 Solanum lycopersicum CoA + chlorogenic acid
-
 ?
2.3.1.99 feruloyl-CoA + quinate condensation at 10% the rate of the reaction with coumaroyl-CoA Solanum lycopersicum CoA + O-feruloylquinate
-
 r
2.3.1.99 additional information enzyme involved in the biosynthesis of chlorogenic acid Solanum lycopersicum ?
-
 ?
2.3.1.99 p-coumaroyl-CoA + shikimate poor substrate Solanum lycopersicum CoA + 5-O-(p-coumaroyl)shikimate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.99 7
-
-
 Solanum lycopersicum