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Literature summary extracted from

  • Taniguchi, N.
    Purification and some properties of gamma-glutamyl transpeptidase from azo dye-induced hepatoma (1974), J. Biochem., 75, 473-480.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 5,5'-dithiobis(2-nitrobenzoate) weak Rattus norvegicus
2.3.2.2 EDTA no inhibition Rattus norvegicus
2.3.2.2 iodoacetamide no inhibition Rattus norvegicus
2.3.2.2 iodoacetate no inhibition Rattus norvegicus
2.3.2.2 N-ethylmaleimide weak Rattus norvegicus
2.3.2.2 p-chloromercuribenzoate no inhibition Rattus norvegicus
2.3.2.2 Zn2+ strong Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 1.1
-
 L-Glu-4-nitroanilide
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 membrane
-
 Rattus norvegicus 16020
-
2.3.2.2 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.2 Ca2+ slight activation Rattus norvegicus
2.3.2.2 Ca2+ activation, transpeptidase Rattus norvegicus
2.3.2.2 Ca2+ not hydrolase Rattus norvegicus
2.3.2.2 Mg2+ slight activation Rattus norvegicus
2.3.2.2 Mg2+ activation, transpeptidase Rattus norvegicus
2.3.2.2 Mg2+ not hydrolase Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 additional information
-
 amino acid composition Rattus norvegicus
2.3.2.2 113000
-
 analytical ultracentrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Rattus norvegicus
-
 male, Donryu
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein 36% carbohydrate Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 solubilized by deoxycholate Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 hepatoma cell azo dye-induced Rattus norvegicus
-
2.3.2.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 181
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.2.2 -22°C, at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is 5-L-Glu-4-nitroanilide Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ir
2.3.2.2 glutathione + acceptor
-
 Rattus norvegicus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.2 58
-
 12 min Rattus norvegicus
2.3.2.2 58
-
 in the presence of glutathione, 0.02 M, at least 60 min stable Rattus norvegicus
2.3.2.2 58
-
 20% of initial activity retained after 60 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 additional information
-
 pI: 3.0 Rattus norvegicus
2.3.2.2 8.8 9
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.2 8.1 9.6 about 70% of maximal activity at pH 8.1, about 75% of maximal activity at pH 9.6 Rattus norvegicus