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Literature summary extracted from

  • Allison, D.
    gamma-Glutamyl transpeptidase: kinetics and mechanism (1985), Methods Enzymol., 113, 419-437.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 hippurate stimulates hydrolase reaction, inhibits transpeptidation Mammalia
2.3.2.2 hippurate stimulates hydrolase reaction, inhibits transpeptidation Mus musculus
2.3.2.2 hippurate stimulates hydrolase reaction, inhibits transpeptidation Rattus norvegicus
2.3.2.2 hippurate stimulates hydrolase reaction, inhibits transpeptidation Ovis aries
2.3.2.2 Maleate
-
 Mammalia
2.3.2.2 Maleate
-
 Mus musculus
2.3.2.2 Maleate
-
 Ovis aries
2.3.2.2 Maleate stimulates hydrolase activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 5,5'-dithiobis(2-nitrobenzoate) no inhibition Mammalia
2.3.2.2 5,5'-dithiobis(2-nitrobenzoate) no inhibition Ovis aries
2.3.2.2 5,5'-dithiobis(2-nitrobenzoate) no inhibition Rattus norvegicus
2.3.2.2 5-glutamylhydrazones
-
 Mammalia
2.3.2.2 5-glutamylhydrazones
-
 Mus musculus
2.3.2.2 5-glutamylhydrazones
-
 Ovis aries
2.3.2.2 5-glutamylhydrazones
-
 Rattus norvegicus
2.3.2.2 5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Mammalia
2.3.2.2 5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Mus musculus
2.3.2.2 5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Ovis aries
2.3.2.2 5-L-glutamyl-2-(2-carboxyphenyl)hydrazine i.e. anthglutin, strong, isolated from Penicillium oxalicum Rattus norvegicus
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Mammalia
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Mus musculus
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Ovis aries
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus
2.3.2.2 Acetazolamide Maleate
-
 Mammalia
2.3.2.2 Acetazolamide Maleate
-
 Mus musculus
2.3.2.2 Acetazolamide Maleate
-
 Ovis aries
2.3.2.2 Acetazolamide Maleate
-
 Rattus norvegicus
2.3.2.2 Bromocresol green
-
 Mammalia
2.3.2.2 Bromocresol green
-
 Mus musculus
2.3.2.2 Bromocresol green
-
 Ovis aries
2.3.2.2 Bromocresol green
-
 Rattus norvegicus
2.3.2.2 iodoacetamide irreversible Mammalia
2.3.2.2 iodoacetamide
-
 Mus musculus
2.3.2.2 iodoacetamide irreversible Ovis aries
2.3.2.2 iodoacetamide irreversible Rattus norvegicus
2.3.2.2 L- or D-Serine/borate formation of a tetrahedral complex at the active site Mammalia
2.3.2.2 L- or D-Serine/borate
-
 Mus musculus
2.3.2.2 L- or D-Serine/borate formation of a tetrahedral complex at the active site Ovis aries
2.3.2.2 L- or D-Serine/borate formation of a tetrahedral complex at the active site Rattus norvegicus
2.3.2.2 L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Mammalia
2.3.2.2 L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Mus musculus
2.3.2.2 L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Ovis aries
2.3.2.2 L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
 Rattus norvegicus
2.3.2.2 L-azaserine
-
 Mammalia
2.3.2.2 L-azaserine
-
 Mus musculus
2.3.2.2 L-azaserine
-
 Ovis aries
2.3.2.2 L-azaserine
-
 Rattus norvegicus
2.3.2.2 additional information
-
 Mammalia
2.3.2.2 additional information
-
 Mus musculus
2.3.2.2 additional information
-
 Ovis aries
2.3.2.2 additional information inhibition by diverse amino acids, overview Rattus norvegicus
2.3.2.2 N-ethylmaleimide weak Mammalia
2.3.2.2 N-ethylmaleimide weak Ovis aries
2.3.2.2 N-ethylmaleimide weak Rattus norvegicus
2.3.2.2 p-chloromercuribenzoate no inhibition Mammalia
2.3.2.2 p-chloromercuribenzoate no inhibition Ovis aries
2.3.2.2 p-chloromercuribenzoate no inhibition Rattus norvegicus
2.3.2.2 phenylhydrazides
-
 Mammalia
2.3.2.2 phenylhydrazides
-
 Mus musculus
2.3.2.2 phenylhydrazides
-
 Ovis aries
2.3.2.2 phenylhydrazides
-
 Rattus norvegicus
2.3.2.2 Sulfobromophthalein derivatives
-
 Mammalia
2.3.2.2 Sulfobromophthalein derivatives
-
 Mus musculus
2.3.2.2 Sulfobromophthalein derivatives
-
 Ovis aries
2.3.2.2 Sulfobromophthalein derivatives
-
 Rattus norvegicus
2.3.2.2 Sulfophthalein derivatives
-
 Mammalia
2.3.2.2 Sulfophthalein derivatives
-
 Mus musculus
2.3.2.2 Sulfophthalein derivatives
-
 Ovis aries
2.3.2.2 Sulfophthalein derivatives
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 additional information
-
 additional information
-
 Mammalia
2.3.2.2 additional information
-
 additional information
-
 Mus musculus
2.3.2.2 additional information
-
 additional information kinetic study Rattus norvegicus
2.3.2.2 additional information
-
 additional information kinetic study Ovis aries
2.3.2.2 additional information
-
 additional information Km values for several amino acids and dipeptides, overview Rattus norvegicus
2.3.2.2 0.005 0.0068 L-Glu-4-nitroanilide
-
 Rattus norvegicus
2.3.2.2 0.029 0.035 L-cystine kidney enzyme Rattus norvegicus
2.3.2.2 0.031
-
 5-D-Glu-4-nitroanilide
-
 Rattus norvegicus
2.3.2.2 0.13 0.18 L-Glu-4-nitroanilide with L-Cys-bis-glycine, rat kidney enzyme Rattus norvegicus
2.3.2.2 0.21 0.32 L-Glu-4-nitroanilide with L-Met-Gly, kidney enzyme Rattus norvegicus
2.3.2.2 0.76
-
 L-Gln rat kidney enzyme Rattus norvegicus
2.3.2.2 1.09
-
 L-cysteinylglycine
-
 Rattus norvegicus
2.3.2.2 1.09
-
 L-cysteinylglycine L-Cys-Gly, rat kidney enzyme Rattus norvegicus
2.3.2.2 2.9 2.96 glycylglycine rat kidney enzyme Rattus norvegicus
2.3.2.2 4 4.7 glycylglycine L-Met, rat kidney enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Mammalia involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Mus musculus involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Rattus norvegicus involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Ovis aries involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Mammalia concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Rattus norvegicus concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Ovis aries concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor peptide + 5-L-glutamyl amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Mammalia
-
-
-
2.3.2.2 Mus musculus
-
-
-
2.3.2.2 Ovis aries
-
-
-
2.3.2.2 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Mammalia
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Mus musculus
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Rattus norvegicus
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Ovis aries
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Rattus norvegicus
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Ovis aries
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 cerebellum
-
 Mus musculus
-
2.3.2.2 kidney
-
 Mammalia
-
2.3.2.2 kidney
-
 Mus musculus
-
2.3.2.2 kidney
-
 Rattus norvegicus
-
2.3.2.2 kidney
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 additional information
-
 activities with diverse substrates Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ stereospecificity Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ imino acids are no substrates Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor Mammalia peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-D-Glu-4-nitroanilide + an amino acid
-
 Rattus norvegicus ?
-
 ?
2.3.2.2 5-D-glutamyl-4-nitroanilide + acceptor
-
 Rattus norvegicus 4-nitroaniline + 5-D-glutamyl-acceptor
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Mammalia 4-nitroaniline + glutathione
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Mus musculus 4-nitroaniline + glutathione
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Rattus norvegicus 4-nitroaniline + glutathione
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
-
 Ovis aries 4-nitroaniline + glutathione
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cystine best acceptor Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-cystine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 a (5-L-glutamyl)-peptide + L-cysteinylglycine
-
 Rattus norvegicus ?
-
 ?
2.3.2.2 glutathione + acceptor
-
 Mammalia cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 glutathione + acceptor
-
 Mus musculus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 glutathione + acceptor
-
 Rattus norvegicus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 glutathione + acceptor
-
 Ovis aries cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
2.3.2.2 glutathione + glutathione autotranspeptidation Mammalia L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
2.3.2.2 glutathione + glutathione autotranspeptidation Mus musculus L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
2.3.2.2 glutathione + glutathione autotranspeptidation Rattus norvegicus L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
2.3.2.2 glutathione + glutathione autotranspeptidation Ovis aries L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
2.3.2.2 glutathione + L-cysteinylglycine
-
 Mammalia L-cysteinylglycine + glutathione
-
 ?
2.3.2.2 glutathione + L-cysteinylglycine
-
 Mus musculus L-cysteinylglycine + glutathione
-
 ?
2.3.2.2 glutathione + L-cysteinylglycine
-
 Rattus norvegicus L-cysteinylglycine + glutathione
-
 ?
2.3.2.2 glutathione + L-cysteinylglycine
-
 Ovis aries L-cysteinylglycine + glutathione
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?
2.3.2.2 additional information
-
 Rattus norvegicus ?
-
 ?
2.3.2.2 additional information the donor substrate determines the acceptor specificity Ovis aries ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Mus musculus
2.3.2.2 37
-
 assay at Rattus norvegicus
2.3.2.2 37
-
 assay at Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8
-
 assay at Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.2 additional information
-
 additional information Ki values for diverse amino acids, overview Mammalia
2.3.2.2 additional information
-
 additional information Ki values for diverse amino acids, overview Mus musculus
2.3.2.2 additional information
-
 additional information Ki values for diverse amino acids, overview Rattus norvegicus