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Literature summary extracted from

  • Okabayashi, K.; Nakano, E.
    Partial purification and properties of citrate synthase from sea urchin eggs (1979), J. Biochem., 85, 1061-1066.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 ADP
-
 Heliocidaris crassispina
2.3.3.16 AMP
-
 Heliocidaris crassispina
2.3.3.16 ATP 44% inhibition at 5 mM Heliocidaris crassispina
2.3.3.16 CaCl2 29% inhibition at 10 mM Heliocidaris crassispina
2.3.3.16 Cations monovalent and divalent Heliocidaris crassispina
2.3.3.16 CTP poor Heliocidaris crassispina
2.3.3.16 GTP poor Heliocidaris crassispina
2.3.3.16 iodoacetamide 10% inhibition at 0.002 mM Heliocidaris crassispina
2.3.3.16 KCl 9% inhibition at 50 mM Heliocidaris crassispina
2.3.3.16 MgCl2 32% inhibition at 10 mM Heliocidaris crassispina
2.3.3.16 MnCl2 56% inhibition at 10 mM Heliocidaris crassispina
2.3.3.16 NaCl 15% inhibition at 50 mM Heliocidaris crassispina
2.3.3.16 p-chloromercuribenzoate 6% loss at 0.002 mM Heliocidaris crassispina
2.3.3.16 TTP poor Heliocidaris crassispina
2.3.3.16 UTP poor Heliocidaris crassispina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0032
-
 oxaloacetate
-
 Heliocidaris crassispina
2.3.3.16 0.033
-
 acetyl-CoA
-
 Heliocidaris crassispina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 100000
-
 gel filtration Heliocidaris crassispina

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Heliocidaris crassispina
-
 sea urchin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 partial Heliocidaris crassispina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.16 egg
-
 Heliocidaris crassispina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 1190
-
 partially purified enzyme Heliocidaris crassispina

Storage Stability

EC Number Storage Stability Organism
2.3.3.16 4°C, phosphate buffer, pH 7.4, 20% loss of activity after 2 weeks Heliocidaris crassispina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Heliocidaris crassispina citrate + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 25
-
 enzyme assay at Heliocidaris crassispina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.16 additional information
-
 oxaloacetate protects against heat denaturation Heliocidaris crassispina
2.3.3.16 50
-
 in presence of oxaloacetate 70% of activity retained after 10 min, in absence of oxaloacetate complete loss of activity, acetyl-CoA does not protect Heliocidaris crassispina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 7.8
-
-
 Heliocidaris crassispina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.3.16 7
-
 unstable below Heliocidaris crassispina