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Literature summary extracted from

  • Iredale, S.E.
    Properties of citrate synthase from Pisum sativum mitochondria (1979), Phytochemistry, 18, 1057.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 5,5'-dithiobis(2-nitrobenzoate) no inhibition Pisum sativum
2.3.3.16 ADP 25% inhibition at 5 mM Pisum sativum
2.3.3.16 AMP 8% inhibition at 5 mM Pisum sativum
2.3.3.16 ATP 50% inhibition at 5 mM, competitive against acetyl-CoA; feed back inhibition Pisum sativum
2.3.3.16 HgCl2 weak inhibition Pisum sativum
2.3.3.16 NADH no effect Pisum sativum
2.3.3.16 p-hydroxymercuribenzoate 60% inhibition at 0.1 mM, protection by oxaloacetate Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.016
-
 oxaloacetate
-
 Pisum sativum
2.3.3.16 0.031
-
 acetyl-CoA
-
 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.16 mitochondrion
-
 Pisum sativum 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Pisum sativum entry step to tricarboxylic acid cycle citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Pisum sativum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.16 epicotyl
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Pisum sativum citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O entry step to tricarboxylic acid cycle Pisum sativum citrate + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 25
-
 enzyme assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8.6
-
-
 Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 5
-
 ATP
-
 Pisum sativum