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Literature summary extracted from

  • Gray, G.S.; Bhattacharjee, J.K.
    Biosynthesis of lysine in Saccharomyces cerevisiae: properties and spectrophotometric determination of homocitrate synthase activity (1976), Can. J. Microbiol., 22, 1664-1667.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.14 1,10-phenanthroline 0.01 mM, 91% inhibition Saccharomyces cerevisiae
2.3.3.14 2,2'-dipyridyl 0.01 mM, 82% inhibition Saccharomyces cerevisiae
2.3.3.14 Cu2+ 0.0001 mM CuSO4, complete inhibition Saccharomyces cerevisiae
2.3.3.14 Hg2+ 0.01 mM HgCl2, complete inhibition Saccharomyces cerevisiae
2.3.3.14 iodoacetic acid 0.01 mM, 71% inhibition Saccharomyces cerevisiae
2.3.3.14 L-Lys 10 mM, 80% inhibition. No inhibition by 10 mM D-Lys Saccharomyces cerevisiae
2.3.3.14 Na2HAsO4 0.01 mM, 67% inhibition Saccharomyces cerevisiae
2.3.3.14 NaF 0.01 mM, 73% inhibition Saccharomyces cerevisiae
2.3.3.14 p-hydroxymercuribenzoate 0.0001 mM, complete inhibition Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.14 Mg2+ 0.002 mM MgCl2 has no effect Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.14 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate
-
 Saccharomyces cerevisiae 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.14 32
-
-
 Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.14 25 40 25°C: about 60% of maximal activity, 40°C: about 50% of maximal activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.14 7.5 8
-
 Saccharomyces cerevisiae