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Literature summary extracted from

  • Lee, E.Y.C.; Braun, J.J.
    Sweet corn phosphorylase: purification and properties (1973), Arch. Biochem. Biophys., 156, 276-286.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.1 glycerol, 10%, stabilizes during purification Zea mays
2.4.1.1 sucrose, 10%, stabilizes during purification Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 2,3-diphosphoglycerate 5 mM, 30% inhibition Zea mays
2.4.1.1 ADPglucose 5 mM, 30% inhibition Zea mays
2.4.1.1 additional information not inhibited by fructose 6-phosphate; not inhibited by GMP; not inhibited by GTP; not inhibited by UMP, CMP, CTP Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 150000
-
 sucrose density gradient ultracentrifugation Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Zea mays
-
 sweet corn, var. Golden Bantam, Iowa Belle 104
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 ammonium sulfate, DEAE-cellulose, hydroxylapatite Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 35 49
-
 Zea mays
2.4.1.1 49
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (maltodextrin)n-1 + glucose 1-phosphate
-
 Zea mays (maltodextrin)n + phosphate
-
 r
2.4.1.1 amylopectin + glucose 1-phosphate best substrate Zea mays amylopectin + phosphate
-
 ?
2.4.1.1 glycogen + glucose 1-phosphate poor substrate Zea mays glycogen + phosphate
-
 ?
2.4.1.1 maltoheptaose + glucose 1-phosphate
-
 Zea mays maltooctaose + phosphate
-
 r
2.4.1.1 maltohexaose + glucose 1-phosphate
-
 Zea mays maltoheptaose + phosphate
-
 r
2.4.1.1 maltooctaose + glucose 1-phosphate
-
 Zea mays maltononaose + phosphate
-
 r
2.4.1.1 maltopentaose + glucose 1-phosphate
-
 Zea mays maltohexaose + phosphate
-
 r
2.4.1.1 maltotetraose + glucose 1-phosphate
-
 Zea mays maltopentaose + phosphate
-
 ir
2.4.1.1 maltotriose + glucose 1-phosphate
-
 Zea mays maltotetraose + phosphate
-
 ir
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate polyglucose primer required Zea mays [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.8
-
 shoulder at pH 6.5 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate requirement Zea mays
2.4.1.1 pyridoxal 5'-phosphate 1 mol/mol subunit Zea mays