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Literature summary extracted from

  • Hamdan, I.; Diopoh, J.
    Purification and physicochemical properties of starch phosphorylase from yam (Dioscorea cayenensis) (1991), Plant Sci., 76, 1-7.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 Ca2+ weak Dioscorea cayenensis
2.4.1.1 EDTA 10 mM, 88% inhibition Dioscorea cayenensis
2.4.1.1 Hg2+ 0.1 mM, complete inhibition Dioscorea cayenensis
2.4.1.1 additional information not inhibited by K+, Na+ Dioscorea cayenensis
2.4.1.1 N-Acetylimidazole glucose 1-phosphate prevents Dioscorea cayenensis
2.4.1.1 p-hydroxymercuribenzoate glucose 1-phosphate prevents Dioscorea cayenensis
2.4.1.1 Zn2+ 0.1 mM, complete inhibition; strong inhibition Dioscorea cayenensis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Dioscorea cayenensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 ammonium sulfate, DEAE-Sephadex, Sephadex G-100, Sepharose 6B Dioscorea cayenensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 tuber
-
 Dioscorea cayenensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 20.75
-
-
 Dioscorea cayenensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 starch + glucose 1-phosphate soluble starch Dioscorea cayenensis starch + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with glucose 6-phosphate, fructose 6-phosphate, and fructose 1,6-diphosphate Dioscorea cayenensis [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate strict specificity for glucose 1-phosphate Dioscorea cayenensis [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 45
-
-
 Dioscorea cayenensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 40
-
 stable below Dioscorea cayenensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.9 6.3
-
 Dioscorea cayenensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.2 7.2 approx. half-maximal activity at pH 5.2 and 7.2 Dioscorea cayenensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.1 5.5 7.5 stable Dioscorea cayenensis