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Literature summary extracted from

  • Weinhausel, A.; Griessler, R.; Krebs, A.; Zipper, P.; Haltrich, D.; Kulbe, K.D.; Nidetzky, B.
    alpha-1,4-D-Glucan phosphorylase of gram-positive Corynebacterium callunae: isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering (1997), Biochem. J., 326, 773-783.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 (NH4)2SO4 50-300 mM, weak inhibition Corynebacterium callunae
2.4.1.1 ADP 5 mM, 8% inhibition of glucan phosphorolysis Corynebacterium callunae
2.4.1.1 AMP 1-20 mM, weak inhibition Corynebacterium callunae
2.4.1.1 ATP 5 mM, 10% inhibition of glucan phosphorolysis Corynebacterium callunae
2.4.1.1 D-glucono-delta-lactone 0.05 mM, 90% inhibition of glucan phosphorolysis Corynebacterium callunae
2.4.1.1 D-glucose 50 mM, 30% inhibition of glucan phosphorolysis Corynebacterium callunae
2.4.1.1 molybdate 5 mM, more than 50% inhibition of glucan phosphorolysis Corynebacterium callunae
2.4.1.1 Na2SO4 50-300 mM, weak inhibition Corynebacterium callunae
2.4.1.1 vanadate 5 mM, more than 50% inhibition of glucan phosphorolysis Corynebacterium callunae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.04
-
 starch phosphorolysis Corynebacterium callunae
2.4.1.1 0.27
-
 glycogen DP14 phosphorolysis Corynebacterium callunae
2.4.1.1 0.47
-
 starch DP24 synthesis Corynebacterium callunae
2.4.1.1 0.5
-
 dextrin DP11 phosphorolysis Corynebacterium callunae
2.4.1.1 1.04
-
 glucose 1-phosphate dextrin DP11 synthesis Corynebacterium callunae
2.4.1.1 1.18
-
 phosphate dextrin DP9 phosphorolysis Corynebacterium callunae
2.4.1.1 1.22
-
 dextrin DP9 phosphorolysis Corynebacterium callunae
2.4.1.1 2.73
-
 maltopentaose phosphorolysis Corynebacterium callunae
2.4.1.1 3.15
-
 maltohexaose phosphorolysis Corynebacterium callunae
2.4.1.1 3.72
-
 maltoheptaose phosphorolysis Corynebacterium callunae
2.4.1.1 4.32
-
 glycogen DP14 synthesis Corynebacterium callunae
2.4.1.1 22.7
-
 arsenate maltohexaose arsenolysis Corynebacterium callunae
2.4.1.1 39.86
-
 maltoheptaose synthesis Corynebacterium callunae
2.4.1.1 46.48
-
 dextrin DP11 synthesis Corynebacterium callunae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 88000
-
 2 * 88000, SDS-PAGE Corynebacterium callunae
2.4.1.1 176000
-
 gel filtration Corynebacterium callunae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Corynebacterium callunae
-
 DSM 20147
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 phenyl-Sepharose, maltodextrin-Sepharose 6B, methyl-column Corynebacterium callunae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate sequential mechanism Corynebacterium callunae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 33.53
-
-
 Corynebacterium callunae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate
-
 Corynebacterium callunae (dextrin)n + phosphate
-
 ?
2.4.1.1 dextrin DP11 + phosphate
-
 Corynebacterium callunae dextrin DP10 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.1 dextrin DP9 + phosphate
-
 Corynebacterium callunae dextrin DP8 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.1 glycogen + glucose 1-phosphate
-
 Corynebacterium callunae glycogen + phosphate
-
 r
2.4.1.1 glycogen DP14 + phosphate
-
 Corynebacterium callunae glycogen DP13 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 maltoheptaose + glucose 1-phosphate
-
 Corynebacterium callunae maltooctaose + phosphate
-
 r
2.4.1.1 maltohexaose + arsenate
-
 Corynebacterium callunae maltopentaose + glucose 1-arsenate
-
 ?
2.4.1.1 maltohexaose + glucose 1-phosphate
-
 Corynebacterium callunae maltoheptaose + phosphate
-
 r
2.4.1.1 maltopentaose + glucose 1-phosphate
-
 Corynebacterium callunae maltohexaose + phosphate
-
 r
2.4.1.1 maltotetraose + glucose 1-phosphate
-
 Corynebacterium callunae maltopentaose + phosphate
-
 ir
2.4.1.1 maltotriose + glucose 1-phosphate
-
 Corynebacterium callunae maltotetraose + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate
-
 Corynebacterium callunae starch + phosphate
-
 r
2.4.1.1 starch DP24 + phosphate
-
 Corynebacterium callunae starch DP23 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Corynebacterium callunae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 88000, SDS-PAGE Corynebacterium callunae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 45
-
 glucan phosphorolysis Corynebacterium callunae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.1 4.9
-
 arsenate maltohexaose arsenolysis Corynebacterium callunae
2.4.1.1 33.8
-
 maltopentaose phosphorolysis Corynebacterium callunae
2.4.1.1 34.5
-
 glycogen DP14 phosphorolysis Corynebacterium callunae
2.4.1.1 38.9
-
 maltohexaose phosphorolysis Corynebacterium callunae
2.4.1.1 41.3
-
 maltoheptaose phosphorolysis Corynebacterium callunae
2.4.1.1 47.3
-
 dextrin DP9 phosphorolysis Corynebacterium callunae
2.4.1.1 50
-
 dextrin DP11 phosphorolysis Corynebacterium callunae
2.4.1.1 50.5
-
 phosphate dextrin DP9 phosphorolysis Corynebacterium callunae
2.4.1.1 69.9
-
 starch phosphorolysis Corynebacterium callunae
2.4.1.1 131
-
 glucose 1-phosphate dextrin DP11 synthesis Corynebacterium callunae
2.4.1.1 187
-
 dextrin DP11 synthesis Corynebacterium callunae
2.4.1.1 283
-
 glycogen DP14 synthesis Corynebacterium callunae
2.4.1.1 300
-
 starch DP24 synthesis Corynebacterium callunae
2.4.1.1 312
-
 maltoheptaose synthesis Corynebacterium callunae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.7 6.1 glucan synthesis Corynebacterium callunae
2.4.1.1 7 7.2 glucan phosphorolysis Corynebacterium callunae

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate 0.87 mol/subunit Corynebacterium callunae