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Literature summary extracted from

  • Sprenger, N.; Bortlik, K.; Brandt, A.; Boller, T.; Wiemken, A.
    Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley (1995), Proc. Natl. Acad. Sci. USA, 92, 11652-11656.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression in Escherichia coli Hordeum vulgare
2.4.1.10 screening of barley cDNA library, cloning, transient functional expression in Nicotiana plumbaginifolia protoplasts under control of cauliflower mosaic virus 35S promotor Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 additional information construction of transgenic Nicotiana plumbaginifolia plants that transiently express the functional levansucrase Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 additional information
-
 amino acid sequence Hordeum vulgare
2.4.1.10 23000
-
 1 * 49000 + 1 * 23000, SDS-PAGE Hordeum vulgare
2.4.1.10 49000
-
 1 * 49000 + 1 * 23000, SDS-PAGE Hordeum vulgare
2.4.1.10 67000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Hordeum vulgare transgenic plants glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Hordeum vulgare biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Hordeum vulgare
-
 2 closely similar isoforms
-
2.4.1.10 Hordeum vulgare
-
 cv. Express
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 partial purification Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n transgenic plants Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 dimer 1 * 49000 + 1 * 23000, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 6-SFT
-
 Hordeum vulgare
2.4.1.10 sucrose:fructan 6-fructosyltransferase
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 27
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 5.75
-
 assay at Hordeum vulgare