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Literature summary extracted from

  • Ibrahim, R.K.; Boulay, B.
    Purification and some properties of UDP-glucose:o-dihydroxycoumarin 7-O-glucosyltransferase from tobacco cell cultures (1980), Plant Sci. Lett., 18, 177-184.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.104 0.05
-
 UDPglucose reaction with esculetin Nicotiana tabacum
2.4.1.104 0.095
-
 daphnetin
-
 Nicotiana tabacum
2.4.1.104 0.11
-
 esculetin
-
 Nicotiana tabacum
2.4.1.104 1.43
-
 scopoletin
-
 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.104 UDP-glucose + 7,8-dihydroxycoumarin Nicotiana tabacum i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin UDP + daphnin
-
 ?
2.4.1.104 UDP-glucose + esculetin Nicotiana tabacum i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin UDP + cichoriin
-
 ?
2.4.1.104 UDP-glucose + scopoletin Nicotiana tabacum i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin UDP + scopolin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.104 Nicotiana tabacum
-
 L. cv. Wisconsin No. 38
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.104 partial Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.104 cell suspension culture
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.104 additional information
-
-
 Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.104 UDP-glucose + 7,8-dihydroxycoumarin i.e. daphnetin Nicotiana tabacum UDP + daphnin
-
 ?
2.4.1.104 UDP-glucose + 7,8-dihydroxycoumarin i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin Nicotiana tabacum UDP + daphnin
-
 ?
2.4.1.104 UDP-glucose + caffeic acid 56% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + ?
-
 ?
2.4.1.104 UDP-glucose + esculetin 95% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + cichoriin
-
 ?
2.4.1.104 UDP-glucose + esculetin i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin Nicotiana tabacum UDP + cichoriin
-
 ?
2.4.1.104 UDP-glucose + hydrangetin 30% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + hydrangin
-
 ?
2.4.1.104 UDP-glucose + protocatechuic acid 12% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + ?
-
 ?
2.4.1.104 UDP-glucose + scopoletin 25% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + scopolin
-
 ?
2.4.1.104 UDP-glucose + scopoletin i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin Nicotiana tabacum UDP + scopolin
-
 ?
2.4.1.104 UDP-glucose + syringic acid 10% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + 4-O-glucosyl syringic acid
-
 ?
2.4.1.104 UDP-glucose + umbelliferone 52% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + skimmin
-
 ?
2.4.1.104 UDP-glucose + vanillic acid 14% of the activity with 7,8-dihydroxycoumarin Nicotiana tabacum UDP + 4-O-glucosylvanillic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.104 7.5
-
-
 Nicotiana tabacum