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Literature summary extracted from

  • Avigad, G.; Milner, Y.
    UDP-Glucose:fructose transglucosylase from suger beet roots (1966), Methods Enzymol., 8, 341-345.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.13 2-mercaptoethanol stabilizes Beta vulgaris
2.4.1.13 EDTA, 0.1 mM, stabilizes Beta vulgaris
2.4.1.13 freeze-thawing inactivates Beta vulgaris
2.4.1.13 high salt concentrations inactivate Beta vulgaris
2.4.1.13 protamin sulfate stabilizes Beta vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.13 AMP
-
 Beta vulgaris
2.4.1.13 ATP
-
 Beta vulgaris
2.4.1.13 Cu2+ at 0.01 mM Beta vulgaris
2.4.1.13 Hg2+ at 0.01 mM Beta vulgaris
2.4.1.13 iodoacetic acid
-
 Beta vulgaris
2.4.1.13 N-ethylmaleimide
-
 Beta vulgaris
2.4.1.13 p-hydroxymercuribenzoate
-
 Beta vulgaris
2.4.1.13 TDP
-
 Beta vulgaris
2.4.1.13 UDP strong Beta vulgaris
2.4.1.13 UMP
-
 Beta vulgaris
2.4.1.13 UTP
-
 Beta vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 0.061 0.094 UDP cosubstrate TDP, pH 6 Beta vulgaris
2.4.1.13 0.3 0.38 UDPglucose pH 7.2 Beta vulgaris
2.4.1.13 0.44 0.9 CDP pH 7.2 Beta vulgaris
2.4.1.13 2.08 3.1 fructose pH 7.2 Beta vulgaris
2.4.1.13 52 290 sucrose pH 6 Beta vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.13 cytosol
-
 Beta vulgaris 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.13 UDP + sucrose Beta vulgaris
-
 UDP-glucose + D-fructose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Beta vulgaris
-
 sugar beet
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13
-
 Beta vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.13 root
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.13 0.91
-
-
 Beta vulgaris

Storage Stability

EC Number Storage Stability Organism
2.4.1.13 marked inactivation upon freezing and thawing Beta vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 ADP-glucose + D-fructose
-
 Beta vulgaris ADP + sucrose
-
 r
2.4.1.13 CDP-glucose + D-fructose
-
 Beta vulgaris CDP + sucrose
-
 ?
2.4.1.13 additional information L-sorbose, 5-keto-D-fructose, D-tagatose, fructose 6-phosphate, levanbiose can replace fructose to a small extent, no glucosyl acceptors are D-xylulose, L-rhamnulose, D-glucoheptulose, D-mannoheptulose, turanose, inulobiose, melibiulose, lactulose, cellobiulose, 3,4-di-O-methylfructose, dihydroxyacetone, pyruvate Beta vulgaris ?
-
 ?
2.4.1.13 TDP-glucose + D-fructose
-
 Beta vulgaris TDP + sucrose
-
 r
2.4.1.13 UDP + sucrose
-
 Beta vulgaris UDP-glucose + D-fructose
-
 r
2.4.1.13 UDP + sucrose poor substrate: GDP Beta vulgaris UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose also substrate: ADP Beta vulgaris UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose CDP is poor substrate Beta vulgaris UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose substrate: TDP Beta vulgaris UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDPglucose + D-fructose
-
 Beta vulgaris UDP + sucrose
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.13 37
-
 t1/2: 5 h, pH 6.5 Beta vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 6 6.5 sucrose cleavage Beta vulgaris
2.4.1.13 7
-
 sucrose synthesis Beta vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.13 6.5
-
 t1/2: 5 h, 37°C Beta vulgaris
2.4.1.13 8
-
 most stable Beta vulgaris