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Literature summary extracted from

  • Maekelae, M.; Mattsson, P.; Schinina, M.E.; Korpela, T.
    Purification and properties of cyclomaltodextrin glucanotransferase from an alkalophilic Bacillus (1988), Biotechnol. Appl. Biochem., 10, 414-427.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.19 synthesis ATCC 21783, enzyme is becoming commercially important since cyclodextrins have found various practical applications Niallia circulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19
-
 Bacillus sp. (in: Bacteria)
2.4.1.19
-
 Niallia circulans
2.4.1.19
-
 Paenibacillus macerans

General Stability

EC Number General Stability Organism
2.4.1.19 ATCC 2178, exceptionally stable Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 58000
-
 strain ATCC 21783, gel filtration Niallia circulans
2.4.1.19 70500
-
 ATCC 21783, SDS-PAGE Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. (in: Bacteria)
-
 1011
-
2.4.1.19 Bacillus sp. (in: Bacteria) 1011
-
 1011
-
2.4.1.19 Klebsiella pneumoniae
-
-
-
2.4.1.19 Niallia circulans
-
 B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
2.4.1.19 Paenibacillus macerans
-
 IAM1243
-
2.4.1.19 Paenibacillus macerans IAM1243
-
 IAM1243
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19
-
 Paenibacillus macerans
2.4.1.19 ATCC 21783 Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.19 1650
-
 ATCC 21783 Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 maltoheptaose + glycosyl acceptor ATCC 21783 Niallia circulans beta-cyclodextrin
-
 ?
2.4.1.19 maltopentaose + glycosyl acceptor ATCC 21783 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 maltotetraose + glycosyl acceptor ATCC 21783 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 maltotriose + glycosyl acceptor ATCC 21783 Niallia circulans beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor ATCC 21783 Niallia circulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) 1011 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans IAM1243 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) alpha-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans alpha-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) 1011 alpha-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans IAM1243 alpha-cyclodextrin
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 4.6
-
 ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 Niallia circulans
2.4.1.19 7
-
-
 Paenibacillus macerans
2.4.1.19 7
-
 ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 Niallia circulans
2.4.1.19 9.5
-
 ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 Niallia circulans