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Literature summary extracted from

  • Tanaka, M.; Muto, N.; Yamamoto, I.
    Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in ascorbic acid 2-O-alpha-glucoside formation (1991), Biochim. Biophys. Acta, 1078, 127-132.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.19 16
-
 alpha-cyclodextrin
-
 Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Geobacillus stearothermophilus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 alpha-cyclodextrin + ascorbic acid
-
 Geobacillus stearothermophilus L-ascorbic acid-2-O-alpha-D-glucoside + L-ascorbic acid-2-O-alpha-D-oligoglucoside
-
 r
2.4.1.19 alpha-cyclodextrin + isoascorbic acid
-
 Geobacillus stearothermophilus L-isoascorbic acid-2-O-alpha-D-glucoside + L-isoascorbic acid-2-O-alpha-D-oligoglucoside
-
 r
2.4.1.19 amylose + glycosyl acceptor
-
 Geobacillus stearothermophilus ?
-
 ?
2.4.1.19 beta-cyclodextrin + glycosyl acceptor
-
 Geobacillus stearothermophilus alpha-cyclodextrin + maltooligosaccharide
-
 r
2.4.1.19 dextrin + glycosyl acceptor
-
 Geobacillus stearothermophilus cyclodextrins
-
 r
2.4.1.19 gamma-cyclodextrin + glycosyl acceptor
-
 Geobacillus stearothermophilus maltooligosaccharide
-
 r
2.4.1.19 glycogen + acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin
-
 ?
2.4.1.19 L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + glycosyl acceptor
-
 Geobacillus stearothermophilus L-ascorbic acid alpha-D-glucoside + D-glucosyl-[glycosyl acceptor]
-
 r
2.4.1.19 L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + H2O
-
 Geobacillus stearothermophilus L-ascorbic acid-2-O-alpha-D-glucoside + D-glucose
-
 r
2.4.1.19 L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + glycosyl acceptor
-
 Geobacillus stearothermophilus L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + D-glucosyl-[glycosyl acceptor]
-
 r
2.4.1.19 L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + H2O
-
 Geobacillus stearothermophilus L-ascorbic acid-(2-O-alpha-D-glucosyl)2 + D-glucose
-
 r
2.4.1.19 L-ascorbic acid-(2-O-alpha-D-glucosyl)4 + glycosyl acceptor
-
 Geobacillus stearothermophilus L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + glucosyl-[glycosyl acceptor]
-
 r
2.4.1.19 L-ascorbic acid-(2-O-alpha-D-glucosyl)4 + H2O
-
 Geobacillus stearothermophilus L-ascorbic acid-(2-O-alpha-D-glucosyl)3 + D-glucose
-
 r
2.4.1.19 L-ascorbic acid-2-O-alpha-D-glucoside + H2O
-
 Geobacillus stearothermophilus L-ascorbic acid + D-glucose
-
 ?
2.4.1.19 maltohexaose + L-ascorbic acid
-
 Geobacillus stearothermophilus L-ascorbic acid-(2-O-alpha-D-glucosyl)6
-
 r
2.4.1.19 maltooligosaccharides + glycosyl acceptor
-
 Geobacillus stearothermophilus cyclodextrins
-
 r
2.4.1.19 maltose + acceptor
-
 Geobacillus stearothermophilus ?
-
 r
2.4.1.19 maltose + ascorbic acid
-
 Geobacillus stearothermophilus L-ascorbic acid-2-O-alpha-D-glucoside + D-glucose
-
 r
2.4.1.19 maltose + glycosyl acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin
-
 r
2.4.1.19 maltotetraose + glycosyl acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin
-
 r
2.4.1.19 maltotriose + glycosyl acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin
-
 r
2.4.1.19 additional information disaccharides are not substrates, except maltose Geobacillus stearothermophilus ?
-
 ?
2.4.1.19 additional information isomaltose, sucrose, melibiose, phenyl-alpha-D-glucoside, cellobiose and lactose are not acceptors, L-ascorbic acid-2-O-phosphate and, L-ascorbic acid-2-O-sulfate are not substrates Geobacillus stearothermophilus ?
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Geobacillus stearothermophilus cyclodextrins
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 70
-
 optimum for L-ascorbic acid-2-O-alpha-D-glucoside formation Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5
-
 optimum pH for L-ascorbic acid-2-O-alpha-D-glucoside formation Geobacillus stearothermophilus